KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
L-3-Methyl-2-oxopentanoate | and 2-oxoisopentanoate, 2-oxoisohexanoate, value below, 30°C, pH 7.5 | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
ferricyanide | stimulates | Bacillus subtilis | |
additional information | no requirement for MgCl2 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
39800 | - |
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
175000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxopentanoate | Bacillus subtilis | - |
CO2 + 2-methylbutanal | - |
? | |
additional information | Bacillus subtilis | essential for the synthesis of branched-chain fatty acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
30°C, pH 7.5 | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate | 5% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | CO2 + succinate semialdehyde | - |
? | |
2-oxoisohexanoate | 38% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | CO2 + isopentanal | - |
? | |
2-oxoisopentanoate | 63% of the activity with L-3-methyl- | Bacillus subtilis | CO2 + isobutanal | - |
? | |
3-methyl-2-oxopentanoate | - |
Bacillus subtilis | CO2 + 2-methylbutanal | - |
? | |
L-3-methyl-2-oxopentanoate | stereospecificity towards the L-isomer | Bacillus subtilis | CO2 + 2-methylbutanal | - |
? | |
additional information | essential for the synthesis of branched-chain fatty acids | Bacillus subtilis | ? | - |
? | |
pyruvate | 25% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | ethanal + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6: about 60% of maximum activity, pH 9: about 55% of maximum activity | Bacillus subtilis |