Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Streptomyces griseochromogenes |
Protein Variants | Comment | Organism |
---|---|---|
M37F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Streptomyces griseochromogenes |
M37W | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Streptomyces griseochromogenes |
M37Y | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Streptomyces griseochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00193 | - |
S-adenosyl-L-methionine | wild type enzyme, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
0.00196 | - |
S-adenosyl-L-methionine | mutant enzyme M37Y, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
0.00204 | - |
S-adenosyl-L-methionine | mutant enzyme M37W, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
0.00215 | - |
S-adenosyl-L-methionine | mutant enzyme M37F, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40200 | - |
gel filtration | Streptomyces griseochromogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | Streptomyces griseochromogenes | - |
S-adenosyl 3-(methylthio)propylamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseochromogenes | Q841K9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and gel filtration | Streptomyces griseochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | - |
Streptomyces griseochromogenes | S-adenosyl 3-(methylthio)propylamine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40200, His6-tagged enzyme, SDS-PAGE | Streptomyces griseochromogenes |
Synonyms | Comment | Organism |
---|---|---|
BlsE | - |
Streptomyces griseochromogenes |
S-Adenosyl-L-methionine decarboxylase | - |
Streptomyces griseochromogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
S-adenosyl-L-methionine | mutant enzyme M37Y, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
0.024 | - |
S-adenosyl-L-methionine | mutant enzyme M37F, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
0.024 | - |
S-adenosyl-L-methionine | mutant enzyme M37W, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
0.027 | - |
S-adenosyl-L-methionine | wild type enzyme, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | the fully reconstituted His6-tagged enzyme contains approximately 6.8 iron equivalents per monomer | Streptomyces griseochromogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
630 | - |
S-adenosyl-L-methionine | mutant enzyme M37Y, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
680 | - |
S-adenosyl-L-methionine | mutant enzyme M37F, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
700 | - |
S-adenosyl-L-methionine | mutant enzyme M37W, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes | |
840 | - |
S-adenosyl-L-methionine | wild type enzyme, in 50 mM HEPES buffer (pH 8.0), at 25°C | Streptomyces griseochromogenes |