Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.50 extracted from

  • Kinch, L.N.; Scott, J.R.; Ullman, B.; Phillips, M.A.
    Cloning and kinetic characterization of the Trypanosoma cruzi S-adenosylmethionine decarboxylase (1999), Mol. Biochem. Parasitol., 101, 1-11.
    View publication on PubMed
Search for more literature for 4.1.1.50

Activating Compound

Activating Compound Comment Organism Structure
cadaverine increase of kcat up to 9fold Trypanosoma cruzi
putrescine increase of kcat up to 9fold Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information addition of putrescine increases Km up to 2-fold Trypanosoma cruzi
0.05
-
 S-adenosyl-L-methionine pH 8.0, 37°C, without putrescine Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 2 * 32000, alpha, + 2 * 10000, beta, SDS-PAGE, subunits derived from autocatalytically cleaved protein Trypanosoma cruzi
32000
-
 2 * 32000, alpha, + 2 * 10000, beta, SDS-PAGE, subunits derived from autocatalytically cleaved protein Trypanosoma cruzi
70000
-
 gel filtration Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi O76240
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification autocatalytic processing of 42000 Da protein to 32000 + 10000 Da Trypanosoma cruzi

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
 Trypanosoma cruzi (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 32000, alpha, + 2 * 10000, beta, SDS-PAGE, subunits derived from autocatalytically cleaved protein Trypanosoma cruzi