Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Bl21-CodonPlus (DE3)-RP cells | Azospirillum brasilense |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with precipitant solution containing 15% PEG4000 (w /v), 10% glycerol (v /v) in 100 mM HEPES buffer pH 7.5, at 20°C | Azospirillum brasilense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, PPDC binds two molecules of Mg2+ per dimer | Azospirillum brasilense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-nitrilotriacetic acid affinity chromatography and Superdex-200 gel filtration | Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indolepyruvate | higher affinity for indolepyruvate than for phenylpyruvate but a 10fold higher specificity for the latter | Azospirillum brasilense | indoleacetaldehyde + CO2 | - |
? | |
additional information | completely inactive toward benzoylformate | Azospirillum brasilense | ? | - |
? | |
Phenylpyruvate | converts phenylpyruvate with a 10fold higher specificity than indolepyruvate despite a slightly higher affinity for the latter | Azospirillum brasilense | Phenylacetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry | Azospirillum brasilense |
Synonyms | Comment | Organism |
---|---|---|
phenyl/indolepyruvate decarboxylase | - |
Azospirillum brasilense |
PPDC | - |
Azospirillum brasilense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent, PPDC binds two molecules of thiamine diphosphate per dimer | Azospirillum brasilense |