Protein Variants | Comment | Organism |
---|---|---|
S120A | 8fold diminution in kcat-value | Saccharomyces cerevisiae |
S121A | 42000fold diminution in kcat-value | Saccharomyces cerevisiae |
S153A | 18fold inflation in Kd-value for Mg-ATP, 35fold inflation in Km-value for Mg-ATP | Saccharomyces cerevisiae |
S155A | 26fold inflation in Km-value for Mg-ATP, 16fold inflation in Km-value for diphosphomevalonate | Saccharomyces cerevisiae |
S36A | limited solubility, impaired binding of fluorescent ATP analogue trinitrophenyl-ATP | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphoglycolylproline | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
ATP | wild-type, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.061 | - |
ATP | ATP in form of MgATP2- | Saccharomyces cerevisiae | |
0.065 | - |
ATP | mutant S36A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.065 | - |
ATP | ATP in form of MgATP2- | Saccharomyces cerevisiae | |
0.095 | - |
5-diphosphomevalonate | mutant S121A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.123 | - |
5-diphosphomevalonate | wild-type, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.267 | - |
5-diphosphomevalonate | mutant S120A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.307 | - |
5-diphosphomevalonate | mutant S36A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.525 | - |
ATP | mutant S120A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.525 | - |
ATP | ATP in form of MgATP2- | Saccharomyces cerevisiae | |
0.944 | - |
5-diphosphomevalonate | mutant S153A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
1.592 | - |
ATP | mutant S155A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
1.592 | - |
ATP | ATP in form of MgATP2- | Saccharomyces cerevisiae | |
1.921 | - |
5-diphosphomevalonate | mutant S155A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
2.172 | - |
ATP | mutant S153A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
2.172 | - |
ATP | ATP in form of MgATP2- | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32377 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00015 | - |
mutant S121A, pH 7.0, 30°C | Saccharomyces cerevisiae |
0.8 | - |
mutant S120A, pH 7.0, 30°C | Saccharomyces cerevisiae |
0.85 | - |
mutant S155A, pH 7.0, 30°C | Saccharomyces cerevisiae |
2.19 | - |
mutant S153A, pH 7.0, 30°C | Saccharomyces cerevisiae |
4.36 | - |
mutant S36A, pH 7.0, 30°C | Saccharomyces cerevisiae |
6.4 | - |
wild-type, pH 7.0, 30°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-diphosphomevalonate | - |
Saccharomyces cerevisiae | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + 5-diphosphomevalonate | - |
Saccharomyces cerevisiae | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000115 | - |
5-diphosphomevalonate | mutant S121A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.61 | - |
5-diphosphomevalonate | mutant S120A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.65 | - |
5-diphosphomevalonate | mutant S155A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
1.68 | - |
5-diphosphomevalonate | mutant S153A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
3.35 | - |
5-diphosphomevalonate | mutant S36A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
4.9 | - |
5-diphosphomevalonate | wild-type, pH 7.0, 30°C | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
diphosphoglycolylproline | mutant S120A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.066 | - |
diphosphoglycolylproline | mutant S36A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.105 | - |
diphosphoglycolylproline | wild-type, pH 7.0, 30°C | Saccharomyces cerevisiae | |
1.429 | - |
diphosphoglycolylproline | mutant S155A, pH 7.0, 30°C | Saccharomyces cerevisiae | |
1.576 | - |
diphosphoglycolylproline | mutant S153A, pH 7.0, 30°C | Saccharomyces cerevisiae |