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Literature summary for 4.1.1.31 extracted from

  • Kai, Y.; Matsumura, H.; Inoue, T.; Terada, K.; Nagara, Y.; Yoshinaga, T.; Kihara, A.; Tsumura, K.; Izui, K.
    Three-dimensional structure of phosphoenolpyruvate carboxylase: a proposed mechanism for allosteric inhibition (1999), Proc. Natl. Acad. Sci. USA, 96, 823-828.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.31

Crystallization (Commentary)

Crystallization (Comment) Organism
PEPC complexed with L-Asp, at 2.8 A resolution Escherichia coli
three-dimensional structure of the enzyme complexed with the allosteric inhibitor L-Asp, determined by X-ray diffraction at 2.8 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
R438C Arg438Cys has an increased tendency to dissociate into dimers. Mutant enzyme Arg703Gly shows a 5fold decreased turnover number compared to the wild type enzyme Escherichia coli
R703G/R703G mutant enzyme Arg703Gly/Arg704Gly shows a 20fold decreased turnover number compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 HCO3- wild type enzyme Escherichia coli
0.19
-
 CO2 wild-type enzyme Escherichia coli
0.19
-
 phosphoenolpyruvate wild-type enzyme Escherichia coli
0.19
-
 phosphoenolpyruvate wild type enzyme, and mutant enzyme Arg703Gly/Arg704Gly Escherichia coli
0.29
-
 phosphoenolpyruvate mutant enzyme Arg703Gly Escherichia coli
0.55
-
 HCO3- mutant enzyme Arg703Gly Escherichia coli
6.5
-
 HCO3- mutant enzyme Arg703Gly/Arg704Gly Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 1.5 mM Escherichia coli
Mg2+ activates Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00864
-
-
Escherichia coli P00864 wild type and mutant enzymes Arg703Gly and Arg703Gly/Arg704Gly
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
 Escherichia coli phosphate + oxaloacetate
-
 ?
phosphoenolpyruvate + HCO3-
-
 Escherichia coli phosphate + oxaloacetate
-
 ?

Synonyms

Synonyms Comment Organism
PEPC
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
540
-
 phosphoenolpyruvate wild-type enzyme Escherichia coli