Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, in 100 mM bicine, pH 8.0, and 1.6 M ammonium sulfate, at 4°C | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
I148A | mutant shows 2% catalytic efficiency compared to the wild type enzyme | Helicobacter pylori |
I148D | mutant shows 0.2% catalytic efficiency compared to the wild type enzyme | Helicobacter pylori |
I148F | mutant shows 15% catalytic efficiency compared to the wild type enzyme | Helicobacter pylori |
I148G | mutant shows 0.5% catalytic efficiency compared to the wild type enzyme | Helicobacter pylori |
I148K | mutant shows 2% catalytic efficiency compared to the wild type enzyme | Helicobacter pylori |
I148L | mutant shows 28% catalytic efficiency compared to the wild type enzyme | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148L, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.11 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148F, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.35 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148A, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.36 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148G, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.36 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148K, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.39 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.72 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148D, in 100 mM Tris, pH 8.0 | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | P56129 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
meso-2,6-Diaminoheptanedioate | - |
Helicobacter pylori | L-Lysine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAPDC | - |
Helicobacter pylori |
meso-diaminopimelate decarboxylase | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148D, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.07 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148G, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.28 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148K, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.33 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148A, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.38 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148L, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
0.68 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme I148F, in 100 mM Tris, pH 8.0 | Helicobacter pylori | |
15.65 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, in 100 mM Tris, pH 8.0 | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Helicobacter pylori |