Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for structure-based drug development | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
ADC expression analysis, expression of the HIs-tagged enzyme in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged unprocessed, native precursor ADC, vapor diffusion hanging drop method, the precipitant solution contains 0.1 M sodium cacodylate, pH 6.5, 1.5 M magnesium sulfate, and 20% w/v PEG 2000, 15 days, cryoprotection by 20% v/v glycerol, X-ray diffraction structure determination and analysis at 2.99 A resolution | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Mycobacterium tuberculosis | the enzyme is a critical regulatory enzyme in the pantothenate biosynthetic pathway | beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is autocatalytically self-processing, overview | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme expression analysis | Mycobacterium tuberculosis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | the enzyme is a critical regulatory enzyme in the pantothenate biosynthetic pathway | Mycobacterium tuberculosis | beta-alanine + CO2 | - |
? | |
L-aspartate | Ser25 is the catalytic residue, self-processing and catalytic mechanism, overview | Mycobacterium tuberculosis | beta-alanine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | two tetramers forming a pseudo fourfold rotational symmetry, the enzyme protein shows a double-psi beta-barrel structure | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Mycobacterium tuberculosis |
L-Aspartate-alpha-decarboxylase | - |
Mycobacterium tuberculosis |
More | the enzyme belongs to a small class of self-cleaving and pyruvoyl-dependent amino acid decarboxylases | Mycobacterium tuberculosis |