Literature summary for 3.9.1.3 extracted from

Ma, R.; Kanders, E.; Sundh, U.B.; Geng, M.; Ek, P.; Zetterqvist, O.; Li, J.P.
Mutational study of human phosphohistidine phosphatase: effect on enzymatic activity (2005), Biochem. Biophys. Res. Commun., 337, 887-891.

Search for more literature for 3.9.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Top10 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H102A	inactive	Homo sapiens
H53A	inactive	Homo sapiens
H81A	the mutant shows wild type activity	Homo sapiens
R45A	the specific activity of the mutant is decreased by one order of magnitude compared to the wild type enzyme	Homo sapiens
R78A	the specific activity of the mutant is decreased by about 30% compared to the wild type enzyme	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14000	-	x * 14000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9NRX4	-	-

Purification (Commentary)

Purification (Comment)	Organism
Mono Q column chromatography	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.06	-	cell lysate, mutant enzyme R45A, pH and temperature not specified in the publication	Homo sapiens
0.2	-	purified mutant enzyme R45A, pH and temperature not specified in the publication	Homo sapiens
0.3	-	cell lysate, mutant enzyme R78A, pH and temperature not specified in the publication	Homo sapiens
0.4	-	cell lysate, mutant enzyme H81A, pH and temperature not specified in the publication	Homo sapiens
0.4	-	cell lysate, wild type enzyme, pH and temperature not specified in the publication	Homo sapiens
0.9	-	purified mutant enzyme R78A, pH and temperature not specified in the publication	Homo sapiens
1.4	-	purified mutant enzyme H81A, pH and temperature not specified in the publication	Homo sapiens
1.8	-	purified wild type enzyme, pH and temperature not specified in the publication	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-L-Ala-N-phospho-L-His-L-Pro-L-Phe 4-nitroanilide + H2O	-	Homo sapiens	succinyl-L-Ala-L-His-L-Pro-L-Phe 4-nitroanilide + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 14000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PHPT1	-	Homo sapiens

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Release 2023.1 (January 2023)