Literature summary for 3.9.1.2 extracted from

Fuhrmann, J.; Subramanian, V.; Thompson, P.R.
Targeting the arginine phosphatase YwlE with a catalytic redox-based inhibitor (2013), ACS Chem. Biol., 8, 2024-2032.

Search for more literature for 3.9.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
C14S	mutation of auxiliary cysteine residue present in the active site, significant resuction in kcat value	Geobacillus stearothermophilus
C9S	mutation of active site cysteine, complete loss of activity	Geobacillus stearothermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
N-{(2S)-1-amino-5-[(2-imino-2,5-dihydro-1,3-selenazol-4-yl)amino]-1-oxopentan-2-yl}benzamide	irreversible inhibition, kinact/KI is 310 per M and min, by inducing disulfide bond formation between the two active site cysteine residues	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
100	-	4-nitrophenyl phosphate	wild-type, pH 7.6, 25°C	Geobacillus stearothermophilus
115	-	4-nitrophenyl phosphate	mutant C14S, pH 7.6, 25°C	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	S0F332	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Geobacillus stearothermophilus	4-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
YwlE	-	Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.19	-	4-nitrophenyl phosphate	mutant C14S, pH 7.6, 25°C	Geobacillus stearothermophilus
1.12	-	4-nitrophenyl phosphate	wild-type, pH 7.6, 25°C	Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00169	-	4-nitrophenyl phosphate	mutant C14S, pH 7.6, 25°C	Geobacillus stearothermophilus
0.011	-	4-nitrophenyl phosphate	wild-type, pH 7.6, 25°C	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)