Literature summary for 3.8.1.3 extracted from
Liu, J.Q.; Kurihara, T.; Ichiyama, S.; Miyagi, M.; Tsunasawa, S.; Kawasaki, H.; Soda, K.; Esaki, N.
Reaction mechanism of fluoroacetate dehalogenase from Moraxella sp. B (1998), J. Biol. Chem., 47, 30897-30902.
No PubMed abstract available
Application
Application |
Comment |
Organism |
agriculture |
detoxification of poisonous plants for animal food |
Moraxella sp. |
Cloned(Commentary)
Cloned (Comment) |
Organism |
mutant expression in Escherichia coli, H1 |
Moraxella sp. |
Protein Variants
Protein Variants |
Comment |
Organism |
H272N |
mutant, no activity, substrate blocks binding pocket |
Moraxella sp. |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
33258 |
- |
2 * 33258, H1 |
Moraxella sp. |
67000 |
- |
gel filtration |
Moraxella sp. |
67000 |
- |
H1 recombinant in E. coli |
Moraxella sp. |
Organism
Organism |
UniProt |
Comment |
Textmining |
Moraxella sp. |
- |
- |
- |
Subunits
Subunits |
Comment |
Organism |
dimer |
2 * 33258, H1 |
Moraxella sp. |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
50 |
- |
recombinant in E.coli |
Moraxella sp. |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
20 |
60 |
70% of initial activity lost after 30 min at 50°C |
Moraxella sp. |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
5 |
10 |
- |
Moraxella sp. |