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Literature summary for 3.8.1.2 extracted from

  • Peng, P.; Zheng, Y.; Koehorst, J.J.; Schaap, P.J.; Stams, A.J.M.; Smidt, H.; Atashgahi, S.
    Concurrent haloalkanoate degradation and chlorate reduction by Pseudomonas chloritidismutans AW-1T (2017), Appl. Environ. Microbiol., 83, e00325-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-haloacid + H2O Stutzerimonas chloritidismutans
-
 D-2-hydroxyacid + halide
-
 ?
L-2-haloacid + H2O Stutzerimonas chloritidismutans AW-1T
-
 D-2-hydroxyacid + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Stutzerimonas chloritidismutans
-
-
-
Stutzerimonas chloritidismutans AW-1T
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
 with D-2-chloropropionate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans
0.39
-
 with 2-chlorobutyrate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans
1.43
-
 with chloroacetate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans
1.48
-
 with D-2-bromopropionate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans
1.5
-
 with DL-2-chloropropionate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans
1.54
-
 with L-2-bromopropionate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans
1.58
-
 with L-2-chloropropionate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans
2.1
-
 with bromoacetate as substrate, at pH 10.5 and 30°C Stutzerimonas chloritidismutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorobutyrate + H2O low activity Stutzerimonas chloritidismutans 2-hydroxybutyrate + chloride
-
 ?
bromoacetate + H2O highest activity Stutzerimonas chloritidismutans hydroxyacetate + bromide
-
 ?
chloroacetate + H2O high activity Stutzerimonas chloritidismutans hydroxyacetate + chloride
-
 ?
D-2-bromopropionate + H2O high activity Stutzerimonas chloritidismutans L-2-hydroxypropionate + bromide
-
 ?
D-2-bromopropionate + H2O high activity Stutzerimonas chloritidismutans AW-1T L-2-hydroxypropionate + bromide
-
 ?
D-2-chloropropionate + H2O very low activity Stutzerimonas chloritidismutans L-2-hydroxypropionate + chloride
-
 ?
D-2-chloropropionate + H2O very low activity Stutzerimonas chloritidismutans AW-1T L-2-hydroxypropionate + chloride
-
 ?
DL-2-chloropropionate + H2O high activity Stutzerimonas chloritidismutans DL-2-hydroxypropionate + chloride
-
 ?
L-2-bromopropionate + H2O high activity Stutzerimonas chloritidismutans D-2-hydroxypropionate + bromide
-
 ?
L-2-chloropropionate + H2O high activity Stutzerimonas chloritidismutans D-2-hydroxypropionate + chloride
-
 ?
L-2-chloropropionate + H2O high activity Stutzerimonas chloritidismutans AW-1T D-2-hydroxypropionate + chloride
-
 ?
L-2-haloacid + H2O
-
 Stutzerimonas chloritidismutans D-2-hydroxyacid + halide
-
 ?
L-2-haloacid + H2O
-
 Stutzerimonas chloritidismutans AW-1T D-2-hydroxyacid + halide
-
 ?
additional information no activity with 4-chlorobutyrate, 3-chloropropionate, 3-bromopropionate, 3-iodopropionate, or 2,3-dichloropropionate Stutzerimonas chloritidismutans ?
-
-
additional information no activity with 4-chlorobutyrate, 3-chloropropionate, 3-bromopropionate, 3-iodopropionate, or 2,3-dichloropropionate Stutzerimonas chloritidismutans AW-1T ?
-
-

Synonyms

Synonyms Comment Organism
L-2-haloacid dehalogenase
-
 Stutzerimonas chloritidismutans
L-DEX
-
 Stutzerimonas chloritidismutans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 about 60% activity 20°C, about 80% activity 25°C, 100% activity at 30-35°C, about 109% activity 40°C Stutzerimonas chloritidismutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Stutzerimonas chloritidismutans