Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant enzyme is constructed by introducing three lysyl residues at positions 11, 176 and 185 of enzyme by site-directed mutagenesis | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MnSO4 | - |
Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-haloalkanoic acid + H2O | Pseudomonas sp. | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | Pseudomonas sp. 113 | - |
D-2-hydroxyalkanoic acid + halide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. 113 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide | Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-haloacid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas sp. 113 | (R)-2-hydroxyacid + halide | - |
? | |
DL-2-bromo-n-butyrate + H2O | - |
Pseudomonas sp. | ? | - |
? | |
DL-2-bromo-n-butyrate + H2O | - |
Pseudomonas sp. 113 | ? | - |
? | |
L-2-chloropropionate + H2O | - |
Pseudomonas sp. | D-lactate + HCl | - |
? | |
L-2-chloropropionate + H2O | - |
Pseudomonas sp. 113 | D-lactate + HCl | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas sp. | D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas sp. 113 | D-2-hydroxyalkanoic acid + halide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas sp. |