BRENDA - Enzyme Database show
show all sequences of 3.7.1.4

Regulatory regions in the rat lactase-phlorizin hydrolase gene that control cell-specific expression

Verhave, M.; Krasinski, S.D.; Christian, S.I.; Van Schaik, S.; Van Den Brink, G.R.; Doting, E.M.; Maas, S.M.; Wolthers, K.C.; Grand, R.J.; Montgomery, R.K.; J. Pediatr. Gastroenterol. Nutr. 39, 275-285 (2004)

Data extracted from this reference:

Application
Application
Commentary
Organism
molecular biology
cell specificty of LPH gene expression depends upon both positive and negative interactions among elements in the first 2kb of the LPH 5'-flanking region, generally positive activity between -74 and -37 bp, a cell-specific negative region between -210 and -95 bp, and additional elements further toward the 5' terminus that confer a highly cell-specific response in reporter activity, potential binding sites for various intestinal transcription factors, binding of HNF3beta at three sites is relevant to LPH expression
Rattus norvegicus
Cloned(Commentary)
Commentary
Organism
transient transfection of deletion constructs in both LPH-expressing Caco-2 and LPH-nonexpressing Hep-G2 cells, and in HeLa cells
Rattus norvegicus
Engineering
Amino acid exchange
Commentary
Organism
additional information
deletions of the LPH 5' flanking sequence and fusion 5' to the human growth hormone reporter gene
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Application (protein specific)
Application
Commentary
Organism
molecular biology
cell specificty of LPH gene expression depends upon both positive and negative interactions among elements in the first 2kb of the LPH 5'-flanking region, generally positive activity between -74 and -37 bp, a cell-specific negative region between -210 and -95 bp, and additional elements further toward the 5' terminus that confer a highly cell-specific response in reporter activity, potential binding sites for various intestinal transcription factors, binding of HNF3beta at three sites is relevant to LPH expression
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
transient transfection of deletion constructs in both LPH-expressing Caco-2 and LPH-nonexpressing Hep-G2 cells, and in HeLa cells
Rattus norvegicus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
deletions of the LPH 5' flanking sequence and fusion 5' to the human growth hormone reporter gene
Rattus norvegicus
Other publictions for EC 3.7.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750528
Han
Discovery and structural anal ...
Mycobacteroides abscessus 103, Mycobacteroides abscessus
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286
1959-1971
2019
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1
1
13
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6
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5
5
732550
Zhou
A comparative study on the met ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Molecules
19
177-203
2014
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13
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735304
Frank
-
Mutational analysis of the C-C ...
Topics Catal.
57
376-384
2014
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752294
Frank
-
Mutational analysis of the C- ...
Top. Catal.
57
376-384
2014
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697434
Lehtimaeki
Adult-type hypolactasia is not ...
Homo sapiens
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1
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Loss of galectin-3 impairs mem ...
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701338
Cramm-Behrens
Apical cargo traverses endosom ...
Canis lupus familiaris
Traffic
9
2206-2220
2008
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Bulhoes
Correlation between lactose ab ...
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1
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1
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687838
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Genotyping of the lactase-phlo ...
Homo sapiens
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12
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673123
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Genotyping of the lactase-phlo ...
Homo sapiens
Clin. Chem.
52
148-151
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-
1
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1
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1
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1
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1
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675574
Kuranuki
Dietary sucrose enhances intes ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
52
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2006
1
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675975
Bosse
Gata4 is essential for the mai ...
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3
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654284
Schoefer
Cloning and Expression of a Ph ...
, wK1
Appl. Environ. Microbiol.
70
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2004
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1
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9
1
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1
3
3
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5
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1
1
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2
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11
2
1
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1
1
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1
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9
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1
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1
3
3
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1
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2
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11
2
1
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1
1
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2
2
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671823
Tseung
Partial amino acid sequence an ...
Homo sapiens, Oryctolagus cuniculus
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2004
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2
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675587
Verhave
Regulatory regions in the rat ...
Rattus norvegicus
J. Pediatr. Gastroenterol. Nutr.
39
275-285
2004
-
1
1
-
1
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1
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Enzymatic hydrolysis of pyrido ...
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1
1
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2
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6
1
1
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2
1
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2
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6
2
3
3
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2
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1
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2
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6
1
1
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2
1
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656110
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Prosequence of human lactase-p ...
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1
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1
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1
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1
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1
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1
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208760
Ramaswamy
Lactase-phlorizin hydrolase co ...
Platyrrhini
Biochim. Biophys. Acta
403
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1975
-
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2
3
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1
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1
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1
1
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12
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1
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2
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3
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1
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1
1
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12
-
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1
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-
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208761
Birkenmeier
Enzymatic properties of rat la ...
Rattus norvegicus
Biochim. Biophys. Acta
350
100-112
1974
7
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1
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1
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1
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7
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Minamikawa
An inducible hydrolase from As ...
Aspergillus niger
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2
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1
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12
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12
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1
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12
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289292
Chatterjee
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Pantoea agglomerans, Pantoea agglomerans Y46
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1969
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1
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1
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1
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