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Literature summary for 3.7.1.25 extracted from

  • Bayly, R.; Diberardino, D.; Hughes, E.; Skurray, R.
    The purification and properties of two isofunctional 2-hydroxy-6-oxohepta-2,4-dienoate hydrolases from Alcaligenes eutrophus strain 345 (1987), J. Gen. Microbiol., 133, 815-822 .
No PubMed abstract available
Search for more literature for 3.7.1.25

Activating Compound

Activating Compound Comment Organism Structure
additional information EDTA shows no significant stimulatory effect Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Cupriavidus necator
Mn2+ the activity of isoform HODHI is decreased by about 50% in the presence of Mn2+ Cupriavidus necator
additional information EDTA shows no significant inhibitory effect Cupriavidus necator
p-chloromercuribenzoate isoform HODHI is inactivated to a much greater extent (90%) by p-chloromercuribenzoate than is isoform HODHII (35%) Cupriavidus necator
Zn2+
-
 Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 2-hydroxymuconic semialdehyde isoform HODHI, at pH 7.6 and 23°C Cupriavidus necator
0.058
-
 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate isoform HODHI, at pH 7.6 and 23°C Cupriavidus necator
0.36
-
 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate isoform HODHII, at pH 7.6 and 23°C Cupriavidus necator
62
-
 2-hydroxymuconic semialdehyde isoform HODHII, at pH 7.6 and 23°C Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ isoform HODHII activity is stimulated to a level about 50% higher when Mn2+ is present Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
 isoform HODHI, gel filtration Cupriavidus necator
116000
-
 isoform HODHII, gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O Cupriavidus necator
-
 (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
 ?
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O Cupriavidus necator ATCC 17707
-
 (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator ATCC 17707
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sephadex G200 gel filtration, DEAE Sepharose column chromatography, and Sephadex G-150 gel filtration (isoform HODHI only) Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Cupriavidus necator (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
 ?
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Cupriavidus necator ATCC 17707 (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
 ?
2-hydroxy-5-methylmuconic semialdehyde + H2O
-
 Cupriavidus necator ?
-
 ?
2-hydroxy-5-methylmuconic semialdehyde + H2O
-
 Cupriavidus necator ATCC 17707 ?
-
 ?
2-hydroxymuconic semialdehyde + H2O
-
 Cupriavidus necator ?
-
 ?
2-hydroxymuconic semialdehyde + H2O
-
 Cupriavidus necator ATCC 17707 ?
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 34400, isoform HODHI, SDS-PAGE Cupriavidus necator
homotrimer 3 * 36700, isoform HODHII, SDS-PAGE Cupriavidus necator

Synonyms

Synonyms Comment Organism
HOD hydrolase
-
 Cupriavidus necator
HODHI isoform Cupriavidus necator
HODHII isoform Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 no activity of isoforms HODHII is present after treatment for 2 min at 45°C and isoform HODHI is inactivated after 4 min at the same temperature Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
5 10 both isoforms are active between pH 5.0 and 10.0. There is a marked decrease in activity above pH 9.0. At pH 7.6, the activity of isoform HODHI and HODHII is about 75-80% of that at pH 8.6 Cupriavidus necator