Any feedback?
Literature summary for 3.7.1.24 extracted from
- Characterization of PhlG, a hydrolase that specifically degrades the antifungal compound 2,4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0 (2006), Appl. Environ. Microbiol., 72, 418-427.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | the potent antimicrobial compound 2,4-diacetylphloroglucinol is a major determinant of biocontrol activity of plant-beneficial Pseudomonas fluorescens CHA0 against root diseases caused by fungal pathogens | Pseudomonas fluorescens |
| additional information | by degrading 2,4-diacetylphloroglucinol to mildly toxic monoacetylphloroglucinol, PhlG may help avoid accumulation of a metabolite that at high levels may become toxic to the producing bacterium, as has been observed for strains CHA0 and F11 | Pseudomonas fluorescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene phlG, located upstream of the phlACBD biosynthetic operon, between the phlF and phlH genes which encode pathway-specific regulators, genetic structure of the operon, functional recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pME8032 | Pseudomonas fluorescens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of the DELTAphlG mutant CHA1091 and DELTAphlA/DELTAphlG double mutant | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by monoacetylphloroglucinol, triacetylphloroglucinol, or salicylate | Pseudomonas fluorescens | |
| pyoluteorin | strong inhibition of the enzyme, another antifungal compound produced by the bacterium | Pseudomonas fluorescens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
2,4-diacetylphloroglucinol | recombinant enzyme, pH 7.0, 30°C | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-diacetylphloroglucinol + H2O | Pseudomonas fluorescens | the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate | 2-acetylphloroglucinol + acetate | - |
? | |
| 2,4-diacetylphloroglucinol + H2O | Pseudomonas fluorescens CHA0 | the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate | 2-acetylphloroglucinol + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | A0A2C9EVE6 | gene phlG | - |
| Pseudomonas fluorescens CHA0 | A0A2C9EVE6 | gene phlG | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)/pME8032 by nickel affinity chromatography | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-diacetylphloroglucinol + H2O | - |
Pseudomonas fluorescens | 2-acetylphloroglucinol + acetate | - |
? | |
| 2,4-diacetylphloroglucinol + H2O | the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate | Pseudomonas fluorescens | 2-acetylphloroglucinol + acetate | - |
? | |
| 2,4-diacetylphloroglucinol + H2O | - |
Pseudomonas fluorescens CHA0 | 2-acetylphloroglucinol + acetate | - |
? | |
| 2,4-diacetylphloroglucinol + H2O | the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate | Pseudomonas fluorescens CHA0 | 2-acetylphloroglucinol + acetate | - |
? | |
| additional information | the PhlG enzyme does not degrade other compounds with structures similar to diacetylphloroglucinol, such as monoacetylphloroglucinol and triacetylphloroglucinol, or salicylate, and 2,6-dihydroxyacetophenone, suggesting strict substrate specificity | Pseudomonas fluorescens | ? | - |
? | |
| additional information | the PhlG enzyme does not degrade other compounds with structures similar to diacetylphloroglucinol, such as monoacetylphloroglucinol and triacetylphloroglucinol, or salicylate, and 2,6-dihydroxyacetophenone, suggesting strict substrate specificity | Pseudomonas fluorescens CHA0 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAPG hydrolase | - |
Pseudomonas fluorescens |
| Phlg | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 16 | 42 | 50% of maximal activity within this range, optimum at 30°C | Pseudomonas fluorescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 33 | - |
2,4-diacetylphloroglucinol | recombinant enzyme, pH 7.0, 30°C | Pseudomonas fluorescens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 10% of maximal activity at pH 7.6, inactive at pH 8.0 | Pseudomonas fluorescens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas fluorescens | expression of gene phlG is not influenced by the substrate 2,4-diacetylphloroglucinol or the degradation product monoacetylphloroglucinol but is subject to positive control by the GacS/GacA two-component system and to negative control by the pathway-specific regulators PhlF and PhlH | down |
| Pseudomonas fluorescens | gene phlG expression regulation study, overview | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | both phloretin hydrolase, EC 3.7.1.4, and 2,4-diacetylphloroglucinol hydrolase show no sequence similarity to other known C-C hydrolase enzymes and contain no motifs typical of the alpha/beta-hydrolase-fold superfamily | Pseudomonas fluorescens |
| malfunction | 2,4-diacetylphloroglucinol added to cultures of a DAPG-negative DELTAphlA mutant of strain CHA0 is completely degraded, and MAPG is temporarily accumulated. In contrast, 2,4-diacetylphloroglucinol is not degraded in cultures of a DELTAphlA/DELTAphlG double mutant | Pseudomonas fluorescens |
| metabolism | the potent antimicrobial compound 2,4-diacetylphloroglucinol is a major determinant of biocontrol activity of plant-beneficial Pseudomonas fluorescens CHA0 against root diseases caused by fungal pathogens | Pseudomonas fluorescens |
| physiological function | the hydrolase is required for specific degradation of the antifungal compound 2,4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0, it catalyzes the conversion of the potent antibiotic DAPG to less-toxic MAPG, with a high specificity for its substrate | Pseudomonas fluorescens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
