Literature summary for 3.6.5.3 extracted from

Vitagliano, L.; Ruggiero, A.; Masullo, M.; Cantiello, P.; Arcari, P.; Zagari, A.
The crystal structure of Sulfolobus solfataricus elongation factor 1alpha in complex with magnesium and GDP (2004), Biochemistry, 43, 6630-6636.

Crystallization (Commentary)

Crystallization (Comment)	Organism
elongation factor 1alpha in complex with Mg2+ (100 mM) and GDP. Elongation factor 1alpha in complex with GDP does not bind Mg2+, when the ion is present in the crystallization medium at moderate concentrations (5 mM). Crystals are grown using PEG 4000 and propan-2-ol as precipitants. Diffraction quality crystals are obtained using microbatch under oil technique at 4°C and a protein concentration of 6 mg/ml	Saccharolobus solfataricus

Crystallization (Comment)

Organism

elongation factor 1alpha in complex with Mg2+ (100 mM) and GDP. Elongation factor 1alpha in complex with GDP does not bind Mg2+, when the ion is present in the crystallization medium at moderate concentrations (5 mM). Crystals are grown using PEG 4000 and propan-2-ol as precipitants. Diffraction quality crystals are obtained using microbatch under oil technique at 4°C and a protein concentration of 6 mg/ml

Saccharolobus solfataricus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	plays a marginal role in the nucleotide exchange process	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Synonyms

Synonyms	Comment	Organism
EF-1alpha	-	Saccharolobus solfataricus
elongation factor 1alpha	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)