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Literature summary for 3.6.4.B6 extracted from

  • Reindl, S.; Ghosh, A.; Williams, G.J.; Lassak, K.; Neiner, T.; Henche, A.L.; Albers, S.V.; Tainer, J.A.
    Insights into FlaI functions in archaeal motor assembly and motility from structures, conformations, and genetics (2013), Mol. Cell., 49, 1069-1082.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B6

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Sulfolobus acidocaldarius

Protein Variants

Protein Variants Comment Organism
DELTA1-224 in strains expressing the mutant enzyme no archaellum is assembled and swimming motility is abolished. Mutant enzyme still exhibits 75% of ATPase activity compared to the full-length FlaI Sulfolobus acidocaldarius
DELTA1-29 strains overexpressing FlaIDELTA1–29 show that 10%–20% of the cell population can assemble archaella, whereas complementation with wild-type FlaI leads to 40%–50% of cells having archaella Sulfolobus acidocaldarius
M69E/I72E/F76E localization to the membrane is significantly reduced compared to that of wild-type Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Sulfolobus acidocaldarius essential role in archaellum assembly ADP + phosphate
-
 ?
ATP + H2O Sulfolobus acidocaldarius DSM 639 essential role in archaellum assembly ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J9L0
-
-
Sulfolobus acidocaldarius DSM 639 Q4J9L0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Sulfolobus acidocaldarius ADP + phosphate
-
 ?
ATP + H2O essential role in archaellum assembly Sulfolobus acidocaldarius ADP + phosphate
-
 ?
ATP + H2O
-
 Sulfolobus acidocaldarius DSM 639 ADP + phosphate
-
 ?
ATP + H2O essential role in archaellum assembly Sulfolobus acidocaldarius DSM 639 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer ATP binding locks the FlaI hexamer into a more symmetrical and less dynamic conformation, which may promote hexamer assembly by stabilizing interactions across adjacent subunits FlaI must be able to assemble into its hexameric state to function as the archaellum-assembly ATPase Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
FlaI
-
 Sulfolobus acidocaldarius
Saci_1173 locus name Sulfolobus acidocaldarius