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Literature summary for 3.6.4.B10 extracted from

  • Roh, S.H.; Kasembeli, M.; Galaz-Montoya, J.G.; Trnka, M.; Lau, W.C.; Burlingame, A.; Chiu, W.; Tweardy, D.J.
    Chaperonin TRiC/CCT modulates the folding and activity of leukemogenic fusion oncoprotein AML1-ETO (2016), J. Biol. Chem., 291, 4732-4741 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B10

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bos taurus
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Bos taurus
-
 ADP + phosphate
-
 ?
ATP + H2O Homo sapiens
-
 ADP + phosphate
-
 ?
additional information Bos taurus chaperonin TRiC/CCT modulates the folding and activity of leukemogenic fusion oncoprotein AML1-ETO.A folding intermediate of AML1-ETO binds to TRiC directly, mainly through its beta-strand rich, DNA-binding domain (AML-(1-175)), with the assistance of HSP70. TRiC contributes to AML1-ETO proteostasis through specific interactions between the oncoprotein's DNA-binding domain ?
-
-
additional information Homo sapiens chaperonin TRiC/CCT modulates the folding and activity of leukemogenic fusion oncoprotein AML1-ETO.A folding intermediate of AML1-ETO binds to TRiC directly, mainly through its beta-strand rich, DNA-binding domain (AML-(1-175)), with the assistance of HSP70. TRiC contributes to AML1-ETO proteostasis through specific interactions between the oncoprotein's DNA-binding domain. The interaction between AML1-ETO and TRiC is transient. HSP70 facilitates the direct association of AML1-ETO with TRiC ?
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-

Organism

Organism UniProt Comment Textmining
Bos taurus Q32L40 AND Q3ZBH0 AND Q3T0K2 AND F1N0E5 AND F1MWD3 AND Q3MHL7 AND Q2NKZ1 AND Q3ZCI9 genes CCT1-5, 6A, 7, and 8 encoding subunits CCT-alpha, CCT-beta, CCT-gamma, CCT-delta, CCT-epsilon, CCT-zeta, CCT-eta, and CCT-theta
-
Homo sapiens P17987 AND P78371 AND P49368 AND P50991 AND P48643 AND P40227 AND Q99832 AND P50990 genes CCT1-8 encoding subunits CCT-alpha, CCT-beta, CCT-gamma, CCT-delta, CCT-epsilon, CCT-zeta-1, CCT-eta, and CCT-theta
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from bovine testes by anion exchange chromatography and gel filtration Bos taurus
native enzyme from HeLa cells by anion exchange chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
additional information TRiC co-precipitated with recombinantly expressed FLAG-tagged AML1-ETO in HEK-293 cells, along with HSP70 and HSP90, while actin is not detected. AML1-ETO induction does not affect cell viability, nor does it change the levels of actin, HSP70, HSP90, or TRiC proteins Homo sapiens
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testis
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Bos taurus ADP + phosphate
-
 ?
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?
additional information chaperonin TRiC/CCT modulates the folding and activity of leukemogenic fusion oncoprotein AML1-ETO.A folding intermediate of AML1-ETO binds to TRiC directly, mainly through its beta-strand rich, DNA-binding domain (AML-(1-175)), with the assistance of HSP70. TRiC contributes to AML1-ETO proteostasis through specific interactions between the oncoprotein's DNA-binding domain Bos taurus ?
-
-
additional information chaperonin TRiC/CCT modulates the folding and activity of leukemogenic fusion oncoprotein AML1-ETO.A folding intermediate of AML1-ETO binds to TRiC directly, mainly through its beta-strand rich, DNA-binding domain (AML-(1-175)), with the assistance of HSP70. TRiC contributes to AML1-ETO proteostasis through specific interactions between the oncoprotein's DNA-binding domain. The interaction between AML1-ETO and TRiC is transient. HSP70 facilitates the direct association of AML1-ETO with TRiC Homo sapiens ?
-
-

Subunits

Subunits Comment Organism
More monomeric subunit CCT1 has a molecular weight of about 60 kDa Homo sapiens

Synonyms

Synonyms Comment Organism
CCT
-
 Bos taurus
CCT
-
 Homo sapiens
TriC
-
 Bos taurus
TriC
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bos taurus
8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function chaperonin TRiC/CCT modulates the folding and activity of leukemogenic fusion oncoprotein AML1-ETO. AML1-ETO is the most common fusion oncoprotein causing acute myeloid leukemia (AML), a disease with a 5-year survival rate of only 24%. AML1-ETO functions as a rogue transcription factor, altering the expression of genes critical for myeloid cell development and differentiation. The biosynthesis and folding of the AML1-ETO protein is facilitated by interaction with the essential eukaryotic chaperonin TRiC (or CCT) Bos taurus
physiological function chaperonin TRiC/CCT modulates the folding and activity of leukemogenic fusion oncoprotein AML1-ETO. AML1-ETO is the most common fusion oncoprotein causing acute myeloid leukemia (AML), a disease with a 5-year survival rate of only 24%. AML1-ETO functions as a rogue transcription factor, altering the expression of genes critical for myeloid cell development and differentiation. The biosynthesis and folding of the AML1-ETO protein is facilitated by interaction with the essential eukaryotic chaperonin TRiC (or CCT) Homo sapiens