Cloned (Comment) | Organism |
---|---|
genes encoding subunits CCT-alpha, CCT-beta, CCT-gamma, CCT-delta, CCT-epsilon, CCT-zeta-1, CCT-eta, and CCT-theta, DNA and amino acid sequence determination, sequences comparisons, quantitative real-time PCR expression analysis at different temperatures, overview. GaTRiC subunits are constitutively expressed at all temperatures with the exception of GaTRiCbeta subunit at 20°C reaching 4.5fold expression compared to optimal growth at 12°C | Glaciozyma antarctica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | three categories of residue substitutions are found in alpha, beta, and gamma subunits: (i) bulky/polar side chains to alanine or valine, (ii) charged residues to alanine, and (iii) isoleucine to valine that is expected to increase intramolecular flexibility within the GaTRiC | Glaciozyma antarctica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Glaciozyma antarctica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Glaciozyma antarctica | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glaciozyma antarctica | A0A1S6LQX4 AND A0A1S6LQU3 AND A0A1S6LQU0 AND A0A1S6LQU6 AND A0A1S6LQU1 AND A0A1S6LQU9 AND A0A1S6LQW6 AND A0A1S6LQW7 | genes encoding subunits CCT-alpha, CCT-beta, CCT-gamma, CCT-delta, CCT-epsilon, CCT-zeta-1, CCT-eta, and CCT-theta | - |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange chromatography and gel filtration | Glaciozyma antarctica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | GaTRiC is consistently expressed at all temperatures | Glaciozyma antarctica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Glaciozyma antarctica | ADP + phosphate | - |
? | |
additional information | GaTRiC acts a chaperonin mediating folding of denatured luciferase to the functional stage | Glaciozyma antarctica | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
heterohexadecamer | TRiC forms a high molecular weight heterohexadecamer, comprised of two eight-membered rings | Glaciozyma antarctica |
Synonyms | Comment | Organism |
---|---|---|
CCT | - |
Glaciozyma antarctica |
chaperonin containing t-complex polypeptide-1 | - |
Glaciozyma antarctica |
GaTRiC | - |
Glaciozyma antarctica |
T-complex protein 1 | - |
Glaciozyma antarctica |
TriC | - |
Glaciozyma antarctica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
refolding/ATPase | Glaciozyma antarctica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
30 | activity range, profile overview | Glaciozyma antarctica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
refolding/ATPase assay at | Glaciozyma antarctica |
General Information | Comment | Organism |
---|---|---|
malfunction | three categories of residue substitutions are found in alpha, beta, and gamma subunits: (i) bulky/polar side chains to alanine or valine, (ii) charged residues to alanine, and (iii) isoleucine to valine that is expected to increase intramolecular flexibility within the GaTRiC. The residue substitutions observed in the built structures possibly affect the hydrophobic, hydrogen bonds, and ionic and aromatic interactions which lead to an increase in structural flexibility | Glaciozyma antarctica |
additional information | structure-functional analysis, the three-dimensional structure of GaTRiC is modeled using the Saccharomyces cerevisiae TRiC structure (PDB ID 4V81) as template. Determination of ionic interactions of GaTRiC residues | Glaciozyma antarctica |
physiological function | the chaperonin containing t-complex polypeptide-1 which is also known as TRiC plays a central role in cellular homeostasis by facilitating the folding of approximately 10% or more of newly synthesized proteins which include tubulins, actins, luciferin, Von Hippel-Lindau disease tumor suppressor (VHL), histone deacetylase 3, and other client proteins. Enzyme GaTRiC is important in cell regulation | Glaciozyma antarctica |