Literature summary for 3.6.4.B10 extracted from
Kim, J.; Shin, E.; Jeon, S.; Kim, Y.; Kim, P.; Lee, C.; Nam, S.
Overexpression, purification, and functional characterization of the group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 (2009), Biotechnol. Bioprocess Eng., 14, 551-558 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
the chaperonin gene PhCpn is amplified by PCR from the genomic DNA, subcloned into pET21a vector, and expressed in three Escherichia coli host cells such as BL21, Rosetta, and Codonplus (DE3)DE3. Among these host cells, Escherichia coli Rosetta shows the highest expression level of recombinant PhCpn at induction with 1 mM isopropyl beta-D-thiogalactoside |
Pyrococcus horikoshii |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Mg2+ |
with the addition of Mg2+ ion protection activity on inorganic phosphatase from thermal inactivation at 85°C and 110°C increases 2fold compared with the addition of enzyme alone |
Pyrococcus horikoshii |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
59600 |
- |
SDS-PAGE |
Pyrococcus horikoshii |
Organism
Organism |
UniProt |
Comment |
Textmining |
Pyrococcus horikoshii |
O57762 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
the recombinant enzyme is purified to 91% by heat-shock treatment and anion-exchange chromatography |
Pyrococcus horikoshii |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
ATP + H2O |
activity of the enzyme as a molecular chaperone is examined using hyperthermophilic inorganic phosphatase from Pyrococcus horikoshii as a model substrate. The enzyme protected the inorganic phosphatase from thermal inactivation at 85°C and 110°C |
Pyrococcus horikoshii |
ADP + phosphate |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 59600, SDS-PAGE |
Pyrococcus horikoshii |
Synonyms
Synonyms |
Comment |
Organism |
More |
ordered locus name: PH0017 |
Pyrococcus horikoshii |
PhCPN |
- |
Pyrococcus horikoshii |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
85 |
- |
assay at |
Pyrococcus horikoshii |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.5 |
- |
assay at |
Pyrococcus horikoshii |