Cloned (Comment) | Organism |
---|---|
gene lmo1722, recombinant expression of His-tagged enzyme in Escherichia coli using a constitutively active promoter | Listeria monocytogenes |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockout of enzyme Lmo1722 decreases the number of mature 70S ribosomes at low temperatures and decreases Listeria monocytogenes motility by downregulating flaA expression | Listeria monocytogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme Lmo1722 is mainly associated with the 50S subunit of the ribosome | Listeria monocytogenes | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | Q8Y6G5 | gene lmo1722 | - |
Listeria monocytogenes EGDe | Q8Y6G5 | gene lmo1722 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by ammonium sulfate fractionation, nickel affinity chromatography, and gel filtration | Listeria monocytogenes |
Synonyms | Comment | Organism |
---|---|---|
Lmo1722 | - |
Listeria monocytogenes |
General Information | Comment | Organism |
---|---|---|
malfunction | an enzyme-defective strain is less motile due to downregulation of the major subunit of the flagellum, FlaA, caused by decreased flaA expression. Absence of helicase Lmo1722 decreases the fraction of 50S ribosomal subunits and mature 70S ribosomes and affects the processing of the 23S precursor rRNA. The ribosomal profile can be restored to wild-type levels in a DELTAlmo1722 strain expressing enzyme Lmo1722. Enzyme Lmo1722 lacking the C-terminus shows a reduced affinity for the 50S and 70S fractions | Listeria monocytogenes |
physiological function | the Listeria RNA helicase Lmo1722 is essential for growth at low temperatures, motility, and rRNA processing and is important for ribosomal maturation, being associated mainly with the 50S subunit of the ribosome. RNA helicase Lmo1722 is required for optimal growth at low temperatures, whereas it is dispensable at 37°C. The C-terminal part of enzyme Lmo1722 is redundant for low-temperature growth, motility, 23S rRNA processing, and appropriate ribosomal maturation, but the C-terminus is important for proper guidance of Lmo1722 to the 50S subunit | Listeria monocytogenes |