Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Aha1p | potent stimulator of the enzyme ATPase activity | Saccharomyces cerevisiae | |
Hch1p | - |
Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
S25P | the mutation of Hsp82p impairs ATPase stimulation by Aha1p. The intrinsic ATPase activity of the mutant is comparable to that of wildtype enzyme | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sba1p | - |
Saccharomyces cerevisiae | |
Sti1p | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.118 | - |
ATP | Hsp82p, in the presence of Aha1p, at pH 7.2, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.133 | - |
ATP | Hsp82p, at pH 7.2, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.466 | - |
ATP | Hsp82p, in the presence of Hch1p, at pH 7.2, temperature not specified in the publication | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Saccharomyces cerevisiae | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex S200 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 90000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
90 kDa heat shock protein | - |
Saccharomyces cerevisiae |
Hsp82p | - |
Saccharomyces cerevisiae |
Hsp90 | - |
Saccharomyces cerevisiae |