Literature summary for 3.6.1.B17 extracted from

Hsu, Y.F.; Chen, Y.C.; Hsiao, Y.C.; Wang, B.J.; Lin, S.Y.; Cheng, W.H.; Jauh, G.Y.; Harada, J.J.; Wang, C.S.
AtRH57, a DEAD-box RNA helicase, is involved in feedback inhibition of glucose-mediated abscisic acid accumulation during seedling development and additively affects pre-ribosomal RNA processing with high glucose (2014), Plant J., 77, 119-135.

Search for more literature for 3.6.1.B17

Cloned(Commentary)

Cloned (Comment)	Organism
gene AtRH57, quantitative RT-PCR enzyme expression analysis, overexpression of His-tagged enzyme in Escherichia coli, transient recombinant expression of EGFP-tagged enzyme in onion cells in the nucleus and nucleolus	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of enzyme-deficient mutant rh57-1, the mutant is affected in the glucose metabolism and shows reduced function of protein synthesis, rh57-1 mutants show resistance to antibiotics, phenotypes, overview	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleolus	-	Arabidopsis thaliana	5730	-
nucleus	-	Arabidopsis thaliana	5634	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q84TG1	gene AtRH57	-
Arabidopsis thaliana Col-0	Q84TG1	gene AtRH57	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the enzyme is expressed in all Arabidopsis organs	Arabidopsis thaliana	-
seedling	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	purified recombinant AtRH57-His protein unwinds double-stranded RNA independently of ATP in vitro, the enzyme AtRH57 might be an ATP-independent RNA helicase	Arabidopsis thaliana	?	-	?
additional information	purified recombinant AtRH57-His protein unwinds double-stranded RNA independently of ATP in vitro, the enzyme AtRH57 might be an ATP-independent RNA helicase	Arabidopsis thaliana Col-0	?	-	?

Synonyms

Synonyms	Comment	Organism
ATP-independent RNA helicase	-	Arabidopsis thaliana
AtRH57	-	Arabidopsis thaliana
DEAD-box RNA helicase	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	the transcript of AtRH57 is significantly induced by abscisic acid, high Glc and salt	up

General Information

General Information	Comment	Organism
evolution	enzyme AtRH57 belongs to class II DEAD-box RNA helicase gene family. AtRH57 contains a DESD sequence in motif II and is thus classified into the DExD group	Arabidopsis thaliana
malfunction	Arabidopsis thaliana T-DNA insertion mutant rh57-1 exhibits hypersensitivity to glucose and abscisic acid. The other two rh57 mutants also show glucose hypersensitivity similar to rh57-1, strongly suggesting that the Glc-hypersensitive feature of these mutants results from mutation of AtRH57. Mutants rh57-1 and rh57-3 display severely impaired seedling growth when grown in Glc concentrations higher than 3%. Transcripts of Glc-responsive genes are altered in rh57 mutants, rh57 mutants exhibit abscisic acid hypersensitivity and enhanced abscisic acid accumulation by Glc. Decreased processing of pre-rRNA products in rh57 mutants. rh57-1 mutants show resistance to antibiotics, e.g. streptomycin. Phenotypes, overview	Arabidopsis thaliana
metabolism	AtRH57 acts in a signaling network downstream of hexokinase 1, HXK1	Arabidopsis thaliana

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Release 2023.1 (January 2023)