Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Rosetta | Pyrococcus horikoshii |
expression in Escherichia coli | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with potassium/sodium tartrate at pH 5.5 | Pyrococcus horikoshii |
sitting-drop vapour-diffusion method with postasium/sodium tartrate as the precipitant at pH 5.5. The crystals belong to space group P3(2)21, with approximate unit-cell parameters a = b = 86.7 A, c = 75.4 A and two monomers in the asymmetric unit | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
strain OT3 | - |
Pyrococcus horikoshii OT-3 | - |
strain OT3 | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
ammonium sulfate fractionation and cation exchange chromatography | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diphosphoglycerate + H2O | - |
Pyrococcus horikoshii | 3-phosphoglycerate + phosphate | - |
? | |
1,3-diphosphoglycerate + H2O | - |
Pyrococcus horikoshii OT-3 | 3-phosphoglycerate + phosphate | - |
? | |
acetyl phosphate + H2O | - |
Pyrococcus horikoshii | acetate + phosphate | - |
? | |
acetyl phosphate + H2O | - |
Pyrococcus horikoshii OT-3 | acetate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Pyrococcus horikoshii | benzoate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Pyrococcus horikoshii OT-3 | benzoate + phosphate | - |
? | |
beta-aspartyl phosphate + H2O | - |
Pyrococcus horikoshii | aspartate + phosphate | - |
? | |
beta-aspartyl phosphate + H2O | - |
Pyrococcus horikoshii OT-3 | aspartate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Pyrococcus horikoshii | carbamate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Pyrococcus horikoshii OT-3 | carbamate + phosphate | - |
? | |
succinyl phosphate + H2O | - |
Pyrococcus horikoshii | succinate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACP | - |
Pyrococcus horikoshii |