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Literature summary for 3.6.1.67 extracted from

  • Hill, S.E.; Nguyen, E.; Ukachukwu, C.U.; Freeman, D.M.; Quirk, S.; Lieberman, R.L.
    Metal ion coordination in the E. coli Nudix hydrolase dihydroneopterin triphosphate pyrophosphatase New clues into catalytic mechanism (2017), PLoS ONE, 12, e0180241 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.67

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and enzyme bound to Co2+ and Ni2+, sitting drop vapor diffusion method, using 35% (w/v) PEG 8000, 0.05 M ammonium sulfate in 50 mM Tris pH 7.5, 1 mM EDTA pH 7.4 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ excellent mimic of Mg2+ Escherichia coli
Mg2+ dependent on Escherichia coli
Ni2+ excellent mimic of Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydroneopterin 3'-triphosphate + H2O Escherichia coli
-
 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and Superdex 75 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydroneopterin 3'-triphosphate + H2O
-
 Escherichia coli 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
DHNTPase
-
 Escherichia coli
dihydroneopterin triphosphate pyrophosphatase
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53.9
-
 melting temperature Escherichia coli