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Literature summary for 3.6.1.60 extracted from

  • Cartwright, J.L.; McLennan, A.G.
    The Saccharomyces cerevisiae YOR163w gene encodes a diadenosine 5', 5"'-P1,P6-hexaphosphate (Ap6A) hydrolase member of the MutT motif (Nudix hydrolase) family (1999), J. Biol. Chem., 274, 8604-8610.
    View publication on PubMed
Search for more literature for 3.6.1.60

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol absolute requirement for a reducing agent, such as dithiothreitol (1 mM) Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Saccharomyces cerevisiae Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Saccharomyces cerevisiae
F- noncompetitive inhibitor Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal activity with 4-10 mM Mg2+ or 0.2 mM Mn2+ Saccharomyces cerevisiae
Mn2+ optimal activity with 4-10 mM Mg2+ or 0.2 mM Mn2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21443
-
 x * 21443, calculation from sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O Saccharomyces cerevisiae the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation adenosine 5'-tetraphosphate + AMP
-
 ?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O Saccharomyces cerevisiae the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation adenosine 5'-tetraphosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q99321
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with P1,P4-bis(5'-adenosyl)tetraphosphate or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols Saccharomyces cerevisiae ?
-
 ?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation Saccharomyces cerevisiae adenosine 5'-tetraphosphate + AMP
-
 ?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O hydrolysis of P1,P5-bis(5'-adenosyl)pentaphosphate yields predominantly AMP and adenosine tetraphosphate (96%) Saccharomyces cerevisiae adenosine 5'-tetraphosphate + AMP
-
 ?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation Saccharomyces cerevisiae adenosine 5'-tetraphosphate + ADP
-
 ?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O hydrolysis in H218O shows that ADP and adenosine 5*-tetraphosphate are produced by attack at Pbeta and AMP and adenosine 5'-pentaphosphate are produced by attack at Palpha (76%) and AMP and adenosine 5'-pentaphosphate are produced by attack at Palpha (24%). P1,P6-bis(5'-adenosyl)hexaphosphate is a 8fold better substrate than P1,P5-bis(5'-adenosyl)pentaphosphate Saccharomyces cerevisiae adenosine 5'-tetraphosphate + ADP
-
 ?

Subunits

Subunits Comment Organism
? x * 21443, calculation from sequence Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.08
-
 F- pH 6.9, 37°C Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.3
-
 pH 6.9, 37°C Saccharomyces cerevisiae Ca2+

Expression

Organism Comment Expression
Saccharomyces cerevisiae expressed in log phase yeast cells up