Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | hydrolysis of UDP | Bos taurus | |
UDP | hydrolysis of GDP | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
ribosome | - |
Bos taurus | 5840 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
calf, gel filtration and glycerol density gradient centrifugation | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | Bos taurus | possible biological function in maintaining the cellular pool of GTP-GDP | 5'-GMP + phosphate | - |
? | |
GDP + H2O | Bos taurus | GTP is an important steric effector in many biological systems, GDP a potent inhibitor: GDPase activity could be involved in preventing potential inhibitory levels of GDP from accumulating | 5'-GMP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
calf | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GDP + H2O = GMP + phosphate | stoichiometry of reaction | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
4000 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | best substrate | Bos taurus | 5'-GMP + phosphate | - |
? | |
GDP + H2O | hydrolyzes free GDP, unable to hydrolyze GDP bound to eIF-2, eukaryotic initiation factor 2, and unable to catalyze the exchange of bound GDP with GTP even in the presence of relatively high molar excess of GTP and Met-tRNA | Bos taurus | 5'-GMP + phosphate | - |
? | |
GDP + H2O | possible biological function in maintaining the cellular pool of GTP-GDP | Bos taurus | 5'-GMP + phosphate | - |
? | |
GDP + H2O | GTP is an important steric effector in many biological systems, GDP a potent inhibitor: GDPase activity could be involved in preventing potential inhibitory levels of GDP from accumulating | Bos taurus | 5'-GMP + phosphate | - |
? | |
additional information | substrate specificity | Bos taurus | ? | - |
? | |
additional information | hydrolyzes free GDP, unable to hydrolyze GDP bound to eIF-2, eukaryotic initiation factor 2, and unable to catalyze the exchange of bound GDP with GTP even in the presence of relatively high molar excess of GTP and Met-tRNA | Bos taurus | ? | - |
? | |
additional information | only GDP and UDP are hydrolyzed, no activity with other nucleoside diphosphates and triphosphates, no activity with CDP, ADP, GTP, ATP | Bos taurus | ? | - |
? | |
UDP + H2O | much lower affinity than for GDP | Bos taurus | UMP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, calf, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bos taurus |