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Literature summary for 3.6.1.39 extracted from

  • Dahlman, N.; Ueckermann, C.
    Properties of four different desoxy-thymidine-5'-triphosphate hydrolyzing enzymes in human serum (1982), Biochem. Int., 5, 185-192.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
dTTP
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 70% activation compared with Mn2+ Homo sapiens
Mg2+ 1 mM, 80% activation compared with Mn2+ Homo sapiens
Mn2+ best activator Homo sapiens
Zn2+ 1 mM, very weak activation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTTP + H2O Homo sapiens
-
dTDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-80°C, 10 mM Tris/HCl, pH 7,8, 5 mM mercaptoethanol, stable on freezing and thawing two times Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCTP + H2O 60% activity compared with dTTP as substrate Homo sapiens dCDP + phosphate
-
?
dTTP + H2O
-
Homo sapiens dTDP + phosphate
-
?
dUTP + H2O 50% activity compared with dTTP as substrate Homo sapiens dUDP + phosphate
-
?
UTP + H2O 20% activity compared with dTTP as substrate Homo sapiens UDP + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 no activity at pH 6, very low activity at pH 9 Homo sapiens