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Literature summary for 3.6.1.27 extracted from

  • Chang, H.Y.; Chou, C.C.; Hsu, M.F.; Wang, A.H.
    Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli (2014), J. Biol. Chem., 289, 18719-18735.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.27

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D111A mutation in membrane-water interface, 100% residual activity Escherichia coli
D150A mutation in membrane-water interface, 64% residual activity Escherichia coli
D43A mutation in aequous interface, 73% residual activity Escherichia coli
E17A mutation within the consensus regions, 26% residiual activity Escherichia coli
E17A/E21A mutation within the consensus regions, mutant is completely inactive Escherichia coli
E194A mutation in aequous interface, 31% residual activity Escherichia coli
E21A mutation within the consensus regions, 40% residiual activity Escherichia coli
E41A mutation in aequous interface, 85% residual activity Escherichia coli
E49A mutation in membrane-water interface, 36% residual activity Escherichia coli
H30A mutation within the consensus regions, mutant is completely inactive Escherichia coli
Q53A mutation in membrane-water interface, 14% residual activity Escherichia coli
R174A mutation within the consensus regions, mutant is completely inactive Escherichia coli
R189A mutation in aequous interface, 11% residual activity Escherichia coli
R261A completely inactive Escherichia coli
S173A mutation within the consensus regions, mutant is completely inactive Escherichia coli
S175A mutation within the consensus regions, 32% residiual activity Escherichia coli
T178A mutation within the consensus regions, mutant is completely inactive Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
bacitracin
-
 Escherichia coli
EDTA 5 mM, complete loss of activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0108
-
 farnesyl diphosphate wild-type, pH 7.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ or Mg2+, absolutely required Escherichia coli
Mg2+ or Ca2+, absolutely required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17388
-
 x * 17388, mass spectrometry Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P60932
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + H2O
-
 Escherichia coli farnesyl phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 17388, mass spectrometry Escherichia coli

Synonyms

Synonyms Comment Organism
UppP
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 farnesyl diphosphate wild-type, pH 7.0, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0078
-
 bacitracin pH 7.0, 37°C Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.033
-
 pH 7.0, 37°C Escherichia coli bacitracin