Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanosine 5'-pentaphosphate | 1 mM, 73% resiudal activity | Mycobacterium tuberculosis | |
guanosine 5'-tetraphosphate | 1 mM, 68% resiudal activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0059 | - |
(polyphosphate)14 | pH 6.8, 37°C | Mycobacterium tuberculosis | |
0.0121 | - |
(polyphosphate)60 | pH 6.8, 37°C | Mycobacterium tuberculosis | |
0.0173 | - |
(polyphosphate)130 | pH 6.8, 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | may partly substitute for Mn2+ | Mycobacterium tuberculosis | |
Mn2+ | required, optimum concentration 1 mM | Mycobacterium tuberculosis | |
additional information | no activity with Ca2+, Co2+, Cu2+ or Fe2+ ions | Mycobacterium tuberculosis | |
Zn2+ | may partly substitute for Mn2+ | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHV5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WHV5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(polyphosphate)130 + H2O | - |
Mycobacterium tuberculosis | (polyphosphate)129 + phosphate | - |
? | |
(polyphosphate)130 + H2O | - |
Mycobacterium tuberculosis H37Rv | (polyphosphate)129 + phosphate | - |
? | |
(polyphosphate)14 + H2O | - |
Mycobacterium tuberculosis | (polyphosphate)13 + phosphate | - |
? | |
(polyphosphate)14 + H2O | - |
Mycobacterium tuberculosis H37Rv | (polyphosphate)13 + phosphate | - |
? | |
(polyphosphate)60 + H2O | - |
Mycobacterium tuberculosis | (polyphosphate)59 + phosphate | - |
? | |
(polyphosphate)60 + H2O | - |
Mycobacterium tuberculosis H37Rv | (polyphosphate)59 + phosphate | - |
? | |
additional information | no substrate: guanosine pentaphosphate. Enzyme hydrolyzes ATP and ADP, but lacks GTPase activities | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no substrate: guanosine pentaphosphate. Enzyme hydrolyzes ATP and ADP, but lacks GTPase activities | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rv0496 | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
(polyphosphate)130 | pH 6.8, 37°C | Mycobacterium tuberculosis | |
7.3 | - |
(polyphosphate)60 | pH 6.8, 37°C | Mycobacterium tuberculosis | |
10.2 | - |
(polyphosphate)14 | pH 6.8, 37°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
387 | - |
(polyphosphate)130 | pH 6.8, 37°C | Mycobacterium tuberculosis | |
603 | - |
(polyphosphate)60 | pH 6.8, 37°C | Mycobacterium tuberculosis | |
1729 | - |
(polyphosphate)14 | pH 6.8, 37°C | Mycobacterium tuberculosis |