Cloned (Comment) | Organism |
---|---|
gene acdS, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta | Amycolatopsis methanolica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Amycolatopsis methanolica | |
1.7 | - |
1-aminocyclopropane-1-carboxylate | pH 8.5, 60°C, recombinant His-tagged enzyme | Amycolatopsis methanolica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
144000 | - |
recombinant His-tagged enzyme, gel filtration | Amycolatopsis methanolica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + H2O | Amycolatopsis methanolica | - |
2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | Amycolatopsis methanolica 239 | - |
2-oxobutanoate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis methanolica | A0A076MQB4 | - |
- |
Amycolatopsis methanolica 239 | A0A076MQB4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta by metal chelate affinity chromatography | Amycolatopsis methanolica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + H2O | - |
Amycolatopsis methanolica | 2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | high substrate specificity of enzyme ACCD | Amycolatopsis methanolica | 2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | - |
Amycolatopsis methanolica 239 | 2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | high substrate specificity of enzyme ACCD | Amycolatopsis methanolica 239 | 2-oxobutanoate + NH3 | - |
? | |
additional information | ACC deaminase activity is detected by the following three methods: 2-oxobutyrate production in the coupled reaction with lactate dehydrogenase and NADH, 2-oxobutyrate production by the colorimetric method, and production of ammonium ions in the coupled reaction with glutamate dehydrogenase | Amycolatopsis methanolica | ? | - |
- |
|
additional information | ACC deaminase activity is detected by the following three methods: 2-oxobutyrate production in the coupled reaction with lactate dehydrogenase and NADH, 2-oxobutyrate production by the colorimetric method, and production of ammonium ions in the coupled reaction with glutamate dehydrogenase | Amycolatopsis methanolica 239 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36000, recombinant His-tagged enzyme, SDS-PAGE | Amycolatopsis methanolica |
Synonyms | Comment | Organism |
---|---|---|
ACCD | - |
Amycolatopsis methanolica |
AcdS | - |
Amycolatopsis methanolica |
AMETH_2787 | locus name | Amycolatopsis methanolica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Amycolatopsis methanolica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
1-aminocyclopropane-1-carboxylate | pH 8.5, 60°C, recombinant His-tagged enzyme | Amycolatopsis methanolica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Amycolatopsis methanolica |
General Information | Comment | Organism |
---|---|---|
physiological function | one of the key mechanisms of the effect of bacteria on plant growth and development is their ability to reduce the level of ethylene due to the activity of 1-aminocyclopropanex021-carboxylate deaminase (ACCD). This enzyme catalyzes the hydrolysis of 1-aminocyclopropanex021-carboxylate (ACC), which is an immediate precursor in ethylene biosynthesis, to 2-oxobutyrate and ammonium ions. ACCDx02possessing bacteria contribute to the enhancement of plant resistance to such negative impacts as drought, soil salinity, heavy metal pollution, and the presence of phytopathogens. Amycolatopsis methanolica is a freex02living soil bacterium, apparently not directly associated with plant surface | Amycolatopsis methanolica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
1-aminocyclopropane-1-carboxylate | pH 8.5, 60°C, recombinant His-tagged enzyme | Amycolatopsis methanolica |