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Literature summary for 3.5.5.7 extracted from

  • Liu, D.; Xi, L.; Han, D.; Dou, K.; Su, S.; Liu, J.
    Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6 (2019), Biotechnol. Lett., 41, 583-589 .
    View publication on PubMed
Search for more literature for 3.5.5.7

Activating Compound

Activating Compound Comment Organism Structure
DTT 1 mM, 1.18fold activation Pannonibacter carbonis
EDTA 1 mM, 1.35fold activation Pannonibacter carbonis
glycerin 1% (v/v), 1.3fold activation Pannonibacter carbonis
Isopropanol 1% (v/v), 1.2fold activation Pannonibacter carbonis
L-cysteine 1 mM, 1.29fold activation Pannonibacter carbonis
L-glutathione 1 mM, 1.27fold activation Pannonibacter carbonis
n-hexane 1% (v/v), 1.1fold activation Pannonibacter carbonis
SDS 1% (v/v), 1.4fold activation Pannonibacter carbonis
Span-80 1% (v/v), 1.2fold activation Pannonibacter carbonis

Application

Application Comment Organism
industry the enzyme is a promising biocatalyst for mild nitrile hydrolysis Pannonibacter carbonis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pannonibacter carbonis

Inhibitors

Inhibitors Comment Organism Structure
BaCl2 5 mM, 35% inhibition Pannonibacter carbonis
CaCl2 5 mM, 34% inhibition Pannonibacter carbonis
DTT 5 mM, 37% inhibition Pannonibacter carbonis
FeCl3 5 mM, 23% inhibition Pannonibacter carbonis
FeSO4 5 mM, 47% inhibition Pannonibacter carbonis
glycerin 5% (v/v), 52% inhibition Pannonibacter carbonis
KCl 5 mM, 58% inhibition Pannonibacter carbonis
MgCl2 5 mM, 18% inhibition Pannonibacter carbonis
MnCl2 5 mM, 50% inhibition Pannonibacter carbonis
n-hexane 5% (v/v), 6% inhibition Pannonibacter carbonis
NaCl 5 mM, 26% inhibition Pannonibacter carbonis
SDS 5% (v/v), 37% inhibition Pannonibacter carbonis
Triton X-100 5% (v/v), 14% inhibition Pannonibacter carbonis
ZnCl2 5 mM, 32% inhibition Pannonibacter carbonis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.31
-
 3-Cyanopyridine pH 7.2, 30°C Pannonibacter carbonis

Metals/Ions

Metals/Ions Comment Organism Structure
AgNO3 1 mM, 1.97fold activation Pannonibacter carbonis
CoCl2 1 mM, 1.08fold activation Pannonibacter carbonis
CuCl2 1 mM, 1.28fold activation Pannonibacter carbonis
HgCl2 1 mM, 3.2fold activation Pannonibacter carbonis
NiCl2 1 mM, 1.67fold activation Pannonibacter carbonis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 SDS-PAGE Pannonibacter carbonis

Organism

Organism UniProt Comment Textmining
Pannonibacter carbonis
-
-
-
Pannonibacter carbonis Q4.6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pannonibacter carbonis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylglutaronitrile + 4 H2O activity is 2.3fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 2-methylglutaric acid + 2 NH3
-
 ?
2-methylglutaronitrile + 4 H2O activity is 2.3fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 2-methylglutaric acid + 2 NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 Pannonibacter carbonis pyridine 3-carboxylic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 Pannonibacter carbonis Q4.6 pyridine 3-carboxylic acid + NH3
-
 ?
3-phenylpropionitrile + 2 H2O activity is 2.15fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 3-phenylpropionic acid + NH3
-
 ?
4-chlorobutyronitrile + 2 H2O activity is 2.44fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 4-chlorobutanoic acid + NH3
-
 ?
acrylonitrile + 2 H2O activity is 2.2fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis acrylic acid + NH3
-
 ?
acrylonitrile + 2 H2O activity is 2.2fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 acrylic acid + NH3
-
 ?
adiponitrile + 4 H2O activity is 2.13fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis adipic acid + 2 NH3
-
 ?
benzonitrile + 2 H2O activity is 2.24fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O activity is 2.24fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 benzoic acid + NH3
-
 ?
dodecanenitrile + 2 H2O activity is 2.46fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis dodecanoic acid + NH3
-
 ?
fumaronitrile + 4 H2O activity is 2.21fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis fumaric acid + 2 NH3
-
 ?
iminodiacetonitrile + 2 H2O activity is 2.34fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis iminodiacetic acid + NH3
-
 ?
additional information the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles Pannonibacter carbonis ?
-
-
additional information the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles Pannonibacter carbonis Q4.6 ?
-
-
pentanenitrile + 2 H2O activity is 2.39fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis pentanoic acid + NH3
-
 ?
sebaconitrile + 4 H2O activity is 1.92fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis sebaconic acid + 2 NH3
-
 ?
succinonitrile + 4 H2O activity is 2.64fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis succinic acid + 2 NH3
-
 ?
thiophene-3-carbonitrile + 2 H2O activity is 2.42fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis thiophene-3-carboxylic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PaCNit
-
 Pannonibacter carbonis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Pannonibacter carbonis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 30°C: about 60% of maximal activity, 90°C: about 45% of maximal activity Pannonibacter carbonis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 48 h, 35% loss of activity Pannonibacter carbonis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pannonibacter carbonis

pH Range

pH Minimum pH Maximum Comment Organism
3 11 pH 3.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity Pannonibacter carbonis