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Literature summary for 3.5.5.7 extracted from
- Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6 (2019), Biotechnol. Lett., 41, 583-589 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | 1 mM, 1.18fold activation | Pannonibacter carbonis |  |
| EDTA | 1 mM, 1.35fold activation | Pannonibacter carbonis | |
| glycerin | 1% (v/v), 1.3fold activation | Pannonibacter carbonis | |
| Isopropanol | 1% (v/v), 1.2fold activation | Pannonibacter carbonis | |
| L-cysteine | 1 mM, 1.29fold activation | Pannonibacter carbonis | |
| L-glutathione | 1 mM, 1.27fold activation | Pannonibacter carbonis | |
| n-hexane | 1% (v/v), 1.1fold activation | Pannonibacter carbonis | |
| SDS | 1% (v/v), 1.4fold activation | Pannonibacter carbonis | |
| Span-80 | 1% (v/v), 1.2fold activation | Pannonibacter carbonis |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the enzyme is a promising biocatalyst for mild nitrile hydrolysis | Pannonibacter carbonis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pannonibacter carbonis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | 5 mM, 35% inhibition | Pannonibacter carbonis | |
| CaCl2 | 5 mM, 34% inhibition | Pannonibacter carbonis | |
| DTT | 5 mM, 37% inhibition | Pannonibacter carbonis | |
| FeCl3 | 5 mM, 23% inhibition | Pannonibacter carbonis | |
| FeSO4 | 5 mM, 47% inhibition | Pannonibacter carbonis | |
| glycerin | 5% (v/v), 52% inhibition | Pannonibacter carbonis | |
| KCl | 5 mM, 58% inhibition | Pannonibacter carbonis | |
| MgCl2 | 5 mM, 18% inhibition | Pannonibacter carbonis | |
| MnCl2 | 5 mM, 50% inhibition | Pannonibacter carbonis | |
| n-hexane | 5% (v/v), 6% inhibition | Pannonibacter carbonis | |
| NaCl | 5 mM, 26% inhibition | Pannonibacter carbonis | |
| SDS | 5% (v/v), 37% inhibition | Pannonibacter carbonis | |
| Triton X-100 | 5% (v/v), 14% inhibition | Pannonibacter carbonis | |
| ZnCl2 | 5 mM, 32% inhibition | Pannonibacter carbonis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.31 | - |
3-Cyanopyridine | pH 7.2, 30°C | Pannonibacter carbonis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 1 mM, 1.97fold activation | Pannonibacter carbonis | |
| CoCl2 | 1 mM, 1.08fold activation | Pannonibacter carbonis | |
| CuCl2 | 1 mM, 1.28fold activation | Pannonibacter carbonis | |
| HgCl2 | 1 mM, 3.2fold activation | Pannonibacter carbonis | |
| NiCl2 | 1 mM, 1.67fold activation | Pannonibacter carbonis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
SDS-PAGE | Pannonibacter carbonis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pannonibacter carbonis | - |
- |
- |
| Pannonibacter carbonis Q4.6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pannonibacter carbonis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylglutaronitrile + 4 H2O | activity is 2.3fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | 2-methylglutaric acid + 2 NH3 | - |
? | |
| 2-methylglutaronitrile + 4 H2O | activity is 2.3fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis Q4.6 | 2-methylglutaric acid + 2 NH3 | - |
? | |
| 3-cyanopyridine + 2 H2O | - |
Pannonibacter carbonis | pyridine 3-carboxylic acid + NH3 | - |
? | |
| 3-cyanopyridine + 2 H2O | - |
Pannonibacter carbonis Q4.6 | pyridine 3-carboxylic acid + NH3 | - |
? | |
| 3-phenylpropionitrile + 2 H2O | activity is 2.15fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | 3-phenylpropionic acid + NH3 | - |
? | |
| 4-chlorobutyronitrile + 2 H2O | activity is 2.44fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | 4-chlorobutanoic acid + NH3 | - |
? | |
| acrylonitrile + 2 H2O | activity is 2.2fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | acrylic acid + NH3 | - |
? | |
| acrylonitrile + 2 H2O | activity is 2.2fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis Q4.6 | acrylic acid + NH3 | - |
? | |
| adiponitrile + 4 H2O | activity is 2.13fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | adipic acid + 2 NH3 | - |
? | |
| benzonitrile + 2 H2O | activity is 2.24fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | activity is 2.24fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis Q4.6 | benzoic acid + NH3 | - |
? | |
| dodecanenitrile + 2 H2O | activity is 2.46fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | dodecanoic acid + NH3 | - |
? | |
| fumaronitrile + 4 H2O | activity is 2.21fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | fumaric acid + 2 NH3 | - |
? | |
| iminodiacetonitrile + 2 H2O | activity is 2.34fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | iminodiacetic acid + NH3 | - |
? | |
| additional information | the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles | Pannonibacter carbonis | ? | - |
- |
|
| additional information | the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles | Pannonibacter carbonis Q4.6 | ? | - |
- |
|
| pentanenitrile + 2 H2O | activity is 2.39fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | pentanoic acid + NH3 | - |
? | |
| sebaconitrile + 4 H2O | activity is 1.92fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | sebaconic acid + 2 NH3 | - |
? | |
| succinonitrile + 4 H2O | activity is 2.64fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | succinic acid + 2 NH3 | - |
? | |
| thiophene-3-carbonitrile + 2 H2O | activity is 2.42fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | thiophene-3-carboxylic acid + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PaCNit | - |
Pannonibacter carbonis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Pannonibacter carbonis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | 30°C: about 60% of maximal activity, 90°C: about 45% of maximal activity | Pannonibacter carbonis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
48 h, 35% loss of activity | Pannonibacter carbonis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pannonibacter carbonis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | pH 3.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity | Pannonibacter carbonis |
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Release 2023.1 (January 2023)
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