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Literature summary for 3.5.5.7 extracted from

  • Kobayashi, M.; Yanaka, N.; Nagasawa, T.; Yamada, H.
    Purification and characterization of a novel nitrilase of Rhodococcus rhodochrous K22 that acts on aliphatic nitriles (1990), J. Bacteriol., 172, 4807-4815.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.7

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione Rhodococcus rhodochrous
NH3 inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione Rhodococcus rhodochrous
PCMB
-
 Rhodococcus rhodochrous

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14
-
 acrylonitrile
-
 Rhodococcus rhodochrous
18.9
-
 Crotonitrile
-
 Rhodococcus rhodochrous

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme contains no metal Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol Rhodococcus rhodochrous
41000
-
 or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol Rhodococcus rhodochrous
604000
-
 equilibrium sedimentation Rhodococcus rhodochrous
650000
-
 HPLC gel filtration Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous K22
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.737
-
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-dicyano-2-butene + H2O 42.6% of the activity with crotononitrile Rhodococcus rhodochrous ?
-
 ?
1-cyclohexeneacetonitrile + H2O 18.2% of the activity with crotononitrile Rhodococcus rhodochrous 1-cyclohexene carboxylate + NH3
-
 ?
1-cyclopenteneacetonitrile + H2O 24.9% of the activity with crotononitrile Rhodococcus rhodochrous 1-cyclopentene carboxylate + NH3
-
 ?
2,4-dicyano-1-butene + H2O 127% of the activity with crotononitrile Rhodococcus rhodochrous ?
-
 ?
2-aminocrotononitrile + H2O 30.3% of the activity with crotononitrile Rhodococcus rhodochrous 2-aminocrotonic acid + NH3
-
 ?
2-aminocrotononitrile + H2O 30.3% of the activity with crotononitrile Rhodococcus rhodochrous K22 2-aminocrotonic acid + NH3
-
 ?
2-chloroacrylonitrile + H2O 47.1% of the activity with crotononitrile Rhodococcus rhodochrous 2-chloroacrylic acid + NH3
-
 ?
2-furonitrile + H2O 52.0% of the activity with crotononitrile Rhodococcus rhodochrous furoic acid + NH3
-
 ?
2-methyl-2-butenenitrile + H2O 14.9% of the activity with crotononitrile Rhodococcus rhodochrous 2-methyl-2-butenoic acid + NH3
-
 ?
2-methyl-3-butenenitrile + H2O 46.8% of the activity with crotononitrile Rhodococcus rhodochrous 2-methyl-3-butenoic acid + NH3
-
 ?
2-pentenenitrile + H2O 6.33% of the activity with crotononitrile Rhodococcus rhodochrous 2-pentenoic acid + NH3
-
 ?
2-thiopheneacetonitrile + H2O 73.5% of the activity with crotononitrile Rhodococcus rhodochrous thiophene-2-carboxylate + NH3
-
 ?
2-thiophenecarbonitrile + H2O 61.1% of the activity with crotononitrile Rhodococcus rhodochrous 2-thiophenecarboxylate + NH3
-
 ?
3,3'-oxydipropionitrile + H2O 29.0% of the activity with crotononitrile Rhodococcus rhodochrous ? + NH3
-
 ?
3-chloropropionitrile + H2O 113% of the activity with crotononitrile Rhodococcus rhodochrous 3-chloropropanoate + NH3
-
 ?
3-cyanopyridine + H2O 7.72% of the activity with crotononitrile Rhodococcus rhodochrous 3-carboxypyridine + NH3
-
 ?
3-ethoxyacrylonitrile + H2O 19.1% of the activity with crotononitrile Rhodococcus rhodochrous 3-ethoxyacrylic acid + NH3
-
 ?
3-ethoxyacrylonitrile + H2O 19.1% of the activity with crotononitrile Rhodococcus rhodochrous K22 3-ethoxyacrylic acid + NH3
-
 ?
3-ethoxybenzonitrile + H2O 21.1% of the activity with crotononitrile Rhodococcus rhodochrous 3-ethoxybenzoate + NH3
-
 ?
3-methoxybenzonitrile 24.8% of the activity with crotononitrile Rhodococcus rhodochrous 3-methoxybenzoate + NH3
-
 ?
3-nitrobenzonitrile + H2O 74.5% of the activity with crotononitrile Rhodococcus rhodochrous 3-nitrobenzoate + NH3
-
 ?
3-pentenenitrile + H2O 132% of the activity with crotononitrile Rhodococcus rhodochrous 3-pentenoic acid + NH3
-
 ?
3-thiopheneacetonitrile + H2O 66.3% of the activity with crotononitrile Rhodococcus rhodochrous thiophene-3-carboxylate + NH3
-
 ?
3-tolunitrile + H2O 67.9% of the activity with crotononitrile Rhodococcus rhodochrous 3-methylbenzenecarboxylic acid + NH3
-
 ?
3-xylylene dicyanide + H2O 14.62% of the activity with crotononitrile Rhodococcus rhodochrous ?
-
 ?
4-chlorobutyronitrile + H2O 116% of the activity with crotononitrile Rhodococcus rhodochrous 4-chlorobutyrate + NH3
-
 ?
4-cyano-1-cyclohexene + H2O 7.03% of the activity with crotononitrile Rhodococcus rhodochrous 1-cyclohexene-4-carboxylate + NH3
-
 ?
4-cyanopyridine + H2O 9.71% of the activity with crotononitrile Rhodococcus rhodochrous 4-carboxypyridine + NH3
-
 ?
acetonitrile + H2O 28.3% of the activity with crotononitrile Rhodococcus rhodochrous acetate + NH3
-
 ?
acrylonitrile + H2O 348% of the activity with crotononitrile Rhodococcus rhodochrous acrylic acid + NH3
-
 ?
acrylonitrile + H2O 348% of the activity with crotononitrile Rhodococcus rhodochrous K22 acrylic acid + NH3
-
 ?
adiponitrile + H2O 110% of the activity with crotononitrile Rhodococcus rhodochrous adipic acid + NH3
-
 ?
benzonitrile + 2 H2O 27.1% of the activity with crotononitrile Rhodococcus rhodochrous benzoate + NH3
-
 ?
butyronitrile + H2O 18.0% of the activity with crotononitrile Rhodococcus rhodochrous butyrate + NH3
-
 ?
capronitrile + H2O 38.7% of the activity with crotononitrile Rhodococcus rhodochrous hexanoate + NH3
-
 ?
chloroacetonitrile + H2O 61.7% of the activity with crotononitrile Rhodococcus rhodochrous chloroacetate + NH3
-
 ?
cinnamonitrile + H2O 31.2% of the activity with crotononitrile Rhodococcus rhodochrous cinnamate + NH3
-
 ?
crotonitrile + H2O
-
 Rhodococcus rhodochrous crotonic acid + NH3
-
 ?
crotonitrile + H2O
-
 Rhodococcus rhodochrous K22 crotonic acid + NH3
-
 ?
cyanoacetic acid ethyl ester + H2O 117% of the activity withcrotononitrile Rhodococcus rhodochrous acetic acid ethyl ester + NH3
-
 ?
cyanopyrazine + H2O 17.3% of the activity with crotononitrile Rhodococcus rhodochrous pyrazine-2-carboxylate + NH3
-
 ?
cyclopentanecarbonitrile + H2O 11.7% of the activity with crotononitrile Rhodococcus rhodochrous cyclopentanoic acid + NH3
-
 ?
cyclopropanecarbonitrile + H2O 22.9% of the activity with crotononitrile Rhodococcus rhodochrous cyclopropanoic acid + NH3
-
 ?
diaminomaleonitrile + H2O 18.6% of the activity with crotononitrile Rhodococcus rhodochrous diaminomaleic acid + NH3
-
 ?
fumaronitrile + H2O 27.4% of the activity with crotononitrile Rhodococcus rhodochrous fumaric acid + NH3
-
 ?
glutaronitrile + H2O 345% of the activity with crotononitrile Rhodococcus rhodochrous glutarate + NH3
-
 ?
iminodiacetonitrile + 2 H2O 261% of the activity with crotononitrile Rhodococcus rhodochrous iminodiacetic acid + 2 NH3
-
 ?
isobutyronitrile + H2O 6.74% of the activity with crotononitrile Rhodococcus rhodochrous isobutyrate + NH3
-
 ?
isocapronitrile + H2O 41.5% of the activity with crotononitrile Rhodococcus rhodochrous isohexanoate + NH3
-
 ?
isophthalonitrile + H2O 66.1% of the activity with crotononitrile Rhodococcus rhodochrous isophthalate + NH3
-
 ?
malononitrile + H2O 45.1% of the activity with crotononitrile Rhodococcus rhodochrous malonic acid + NH3
-
 ?
methacrylonitrile + H2O 143% of the activity with crotononitrile Rhodococcus rhodochrous methacrylic acid + NH3
-
 ?
methacrylonitrile + H2O 143% of the activity with crotononitrile Rhodococcus rhodochrous K22 methacrylic acid + NH3
-
 ?
methoxyacetonitrile + H2O 37.4% of the activity with crotononitrile Rhodococcus rhodochrous methoxyacetate + NH3
-
 ?
N-methyl-beta-alaninenitrile + H2O 11.4% of the activity with crotononitrile Rhodococcus rhodochrous N-methyl-beta-Ala + NH3
-
 ?
phenylacetonitrile + 2 H2O 27.3% of the activity with crotononitrile Rhodococcus rhodochrous phenylacetate + NH3
-
 ?
pimelonitrile + H2O 27.3% of the activity with crotononitrile Rhodococcus rhodochrous pimelic acid + NH3
-
 ?
piperonylonitrile + H2O 6.8% of the activity with crotononitrile Rhodococcus rhodochrous (3,4-methylenedioxy)benzoate + NH3
-
 ?
propionitrile + H2O 12.7% of the activity with crotononitrile Rhodococcus rhodochrous propionate + NH3
-
 ?
sebaconitrile + H2O 15.7% of the activity with crotononitrile Rhodococcus rhodochrous ? + NH3
-
 ?
suberonitrile + H2O 21.4% of the activity with crotononitrile Rhodococcus rhodochrous decanedioic acid + NH3
-
 ?
succinonitrile + H2O 271% of the activity with crotononitrile Rhodococcus rhodochrous succinate + NH3
-
 ?
valeronitrile + H2O 40.8% of the activity with crotononitrile Rhodococcus rhodochrous pentanoate + NH3
-
 ?

Subunits

Subunits Comment Organism
hexadecamer or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol Rhodococcus rhodochrous
pentadecamer or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol Rhodococcus rhodochrous

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhodococcus rhodochrous

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 40°C: about 40% of maximal activity, 55°C: about 60% of maximal activity, crotonitrile Rhodococcus rhodochrous

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 pH 7.5, 1 h, stable Rhodococcus rhodochrous
30
-
 pH 7.5, 1 h, 6% loss of activity Rhodococcus rhodochrous
35
-
 pH 7.5, 1 h, 8% loss of activity Rhodococcus rhodochrous
40
-
 pH 7.5, 1 h, 13% loss of activity Rhodococcus rhodochrous
45
-
 pH 7.5, 1 h, 72% loss of activity Rhodococcus rhodochrous
50
-
 pH 7.5, 1 h, 93% loss of activity Rhodococcus rhodochrous
55
-
 pH 7.5, 1 h, 100% loss of activity Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rhodococcus rhodochrous

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 25°C, 45 min, less than 30% loss of activity Rhodococcus rhodochrous
10
-
 25°C, 45 min, 40% loss of activity Rhodococcus rhodochrous