BRENDA - Enzyme Database show
show all sequences of 3.5.5.7

Purification and characterization of a novel nitrilase of Rhodococcus rhodochrous K22 that acts on aliphatic nitriles

Kobayashi, M.; Yanaka, N.; Nagasawa, T.; Yamada, H.; J. Bacteriol. 172, 4807-4815 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
HgCl2
inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione
Rhodococcus rhodochrous
NH3
inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione
Rhodococcus rhodochrous
PCMB
-
Rhodococcus rhodochrous
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14
-
acrylonitrile
-
Rhodococcus rhodochrous
18.9
-
Crotonitrile
-
Rhodococcus rhodochrous
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
enzyme contains no metal
Rhodococcus rhodochrous
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol; or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol
Rhodococcus rhodochrous
604000
-
equilibrium sedimentation
Rhodococcus rhodochrous
650000
-
HPLC gel filtration
Rhodococcus rhodochrous
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous K22
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.737
-
-
Rhodococcus rhodochrous
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-dicyano-2-butene + H2O
42.6% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
?
-
-
-
-
1-cyclohexeneacetonitrile + H2O
18.2% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
1-cyclohexene carboxylate + NH3
-
-
-
-
1-cyclopenteneacetonitrile + H2O
24.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
1-cyclopentene carboxylate + NH3
-
-
-
-
2,4-dicyano-1-butene + H2O
127% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
?
-
-
-
-
2-aminocrotononitrile + H2O
30.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-aminocrotonic acid + NH3
-
-
-
-
2-aminocrotononitrile + H2O
30.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
2-aminocrotonic acid + NH3
-
-
-
-
2-chloroacrylonitrile + H2O
47.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-chloroacrylic acid + NH3
-
-
-
-
2-furonitrile + H2O
52.0% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
furoic acid + NH3
-
-
-
-
2-methyl-2-butenenitrile + H2O
14.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-methyl-2-butenoic acid + NH3
-
-
-
-
2-methyl-3-butenenitrile + H2O
46.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-methyl-3-butenoic acid + NH3
-
-
-
-
2-pentenenitrile + H2O
6.33% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-pentenoic acid + NH3
-
-
-
-
2-thiopheneacetonitrile + H2O
73.5% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
thiophene-2-carboxylate + NH3
-
-
-
-
2-thiophenecarbonitrile + H2O
61.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-thiophenecarboxylate + NH3
-
-
-
-
3,3'-oxydipropionitrile + H2O
29.0% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
? + NH3
-
-
-
-
3-chloropropionitrile + H2O
113% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-chloropropanoate + NH3
-
-
-
-
3-cyanopyridine + H2O
7.72% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-carboxypyridine + NH3
-
-
-
-
3-ethoxyacrylonitrile + H2O
19.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-ethoxyacrylic acid + NH3
-
-
-
-
3-ethoxyacrylonitrile + H2O
19.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
3-ethoxyacrylic acid + NH3
-
-
-
-
3-ethoxybenzonitrile + H2O
21.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-ethoxybenzoate + NH3
-
-
-
-
3-methoxybenzonitrile
24.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-methoxybenzoate + NH3
-
-
-
-
3-nitrobenzonitrile + H2O
74.5% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-nitrobenzoate + NH3
-
-
-
-
3-pentenenitrile + H2O
132% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-pentenoic acid + NH3
-
-
-
-
3-thiopheneacetonitrile + H2O
66.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
thiophene-3-carboxylate + NH3
-
-
-
-
3-tolunitrile + H2O
67.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-methylbenzenecarboxylic acid + NH3
-
-
-
-
3-xylylene dicyanide + H2O
14.62% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
?
-
-
-
-
4-chlorobutyronitrile + H2O
116% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
4-chlorobutyrate + NH3
-
-
-
-
4-cyano-1-cyclohexene + H2O
7.03% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
1-cyclohexene-4-carboxylate + NH3
-
-
-
-
4-cyanopyridine + H2O
9.71% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
4-carboxypyridine + NH3
-
-
-
-
acetonitrile + H2O
28.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
acetate + NH3
-
-
-
-
acrylonitrile + H2O
348% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
acrylic acid + NH3
-
-
-
-
acrylonitrile + H2O
348% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
acrylic acid + NH3
-
-
-
-
adiponitrile + H2O
110% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
adipic acid + NH3
-
-
-
-
benzonitrile + H2O
27.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
benzoate + NH3
-
-
-
-
butyronitrile + H2O
18.0% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
butyrate + NH3
-
-
-
-
capronitrile + H2O
38.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
hexanoate + NH3
-
-
-
-
chloroacetonitrile + H2O
61.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
chloroacetate + NH3
-
-
-
-
cinnamonitrile + H2O
31.2% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
cinnamate + NH3
-
-
-
-
crotonitrile + H2O
-
32506
Rhodococcus rhodochrous
crotonic acid + NH3
-
32506
Rhodococcus rhodochrous
-
crotonitrile + H2O
-
32506
Rhodococcus rhodochrous K22
crotonic acid + NH3
-
32506
Rhodococcus rhodochrous K22
-
cyanoacetic acid ethyl ester + H2O
117% of the activity withcrotononitrile
32506
Rhodococcus rhodochrous
acetic acid ethyl ester + NH3
-
-
-
-
cyanopyrazine + H2O
17.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
pyrazine-2-carboxylate + NH3
-
-
-
-
cyclopentanecarbonitrile + H2O
11.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
cyclopentanoic acid + NH3
-
-
-
-
cyclopropanecarbonitrile + H2O
22.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
cyclopropanoic acid + NH3
-
-
-
-
diaminomaleonitrile + H2O
18.6% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
diaminomaleic acid + NH3
-
-
-
-
fumaronitrile + H2O
27.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
fumaric acid + NH3
-
-
-
-
glutaronitrile + H2O
345% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
glutarate + NH3
-
-
-
-
iminodiacetonitrile + 2 H2O
261% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
iminodiacetic acid + 2 NH3
-
-
-
-
isobutyronitrile + H2O
6.74% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
isobutyrate + NH3
-
-
-
-
isocapronitrile + H2O
41.5% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
isohexanoate + NH3
-
-
-
-
isophthalonitrile + H2O
66.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
isophthalate + NH3
-
-
-
-
malononitrile + H2O
45.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
malonic acid + NH3
-
-
-
-
methacrylonitrile + H2O
143% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
methacrylic acid + NH3
-
-
-
-
methacrylonitrile + H2O
143% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
methacrylic acid + NH3
-
-
-
-
methoxyacetonitrile + H2O
37.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
methoxyacetate + NH3
-
-
-
-
N-methyl-beta-alaninenitrile + H2O
11.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
N-methyl-beta-Ala + NH3
-
-
-
-
phenylacetonitrile + H2O
27.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
phenylacetate + NH3
-
-
-
-
pimelonitrile + H2O
27.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
pimelic acid + NH3
-
-
-
-
piperonylonitrile + H2O
6.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
(3,4-methylenedioxy)benzoate + NH3
-
-
-
-
propionitrile + H2O
12.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
propionate + NH3
-
-
-
-
sebaconitrile + H2O
15.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
? + NH3
-
-
-
-
suberonitrile + H2O
21.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
decanedioic acid + NH3
-
-
-
-
succinonitrile + H2O
271% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
succinate + NH3
-
-
-
-
valeronitrile + H2O
40.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
pentanoate + NH3
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexadecamer
or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol
Rhodococcus rhodochrous
pentadecamer
or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol
Rhodococcus rhodochrous
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Rhodococcus rhodochrous
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
55
40°C: about 40% of maximal activity, 55°C: about 60% of maximal activity, crotonitrile
Rhodococcus rhodochrous
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
pH 7.5, 1 h, stable
Rhodococcus rhodochrous
30
-
pH 7.5, 1 h, 6% loss of activity
Rhodococcus rhodochrous
35
-
pH 7.5, 1 h, 8% loss of activity
Rhodococcus rhodochrous
40
-
pH 7.5, 1 h, 13% loss of activity
Rhodococcus rhodochrous
45
-
pH 7.5, 1 h, 72% loss of activity
Rhodococcus rhodochrous
50
-
pH 7.5, 1 h, 93% loss of activity
Rhodococcus rhodochrous
55
-
pH 7.5, 1 h, 100% loss of activity
Rhodococcus rhodochrous
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Rhodococcus rhodochrous
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
25°C, 45 min, less than 30% loss of activity
Rhodococcus rhodochrous
10
-
25°C, 45 min, 40% loss of activity
Rhodococcus rhodochrous
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
HgCl2
inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione
Rhodococcus rhodochrous
NH3
inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione
Rhodococcus rhodochrous
PCMB
-
Rhodococcus rhodochrous
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14
-
acrylonitrile
-
Rhodococcus rhodochrous
18.9
-
Crotonitrile
-
Rhodococcus rhodochrous
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
enzyme contains no metal
Rhodococcus rhodochrous
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol; or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol
Rhodococcus rhodochrous
604000
-
equilibrium sedimentation
Rhodococcus rhodochrous
650000
-
HPLC gel filtration
Rhodococcus rhodochrous
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.737
-
-
Rhodococcus rhodochrous
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-dicyano-2-butene + H2O
42.6% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
?
-
-
-
-
1-cyclohexeneacetonitrile + H2O
18.2% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
1-cyclohexene carboxylate + NH3
-
-
-
-
1-cyclopenteneacetonitrile + H2O
24.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
1-cyclopentene carboxylate + NH3
-
-
-
-
2,4-dicyano-1-butene + H2O
127% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
?
-
-
-
-
2-aminocrotononitrile + H2O
30.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-aminocrotonic acid + NH3
-
-
-
-
2-aminocrotononitrile + H2O
30.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
2-aminocrotonic acid + NH3
-
-
-
-
2-chloroacrylonitrile + H2O
47.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-chloroacrylic acid + NH3
-
-
-
-
2-furonitrile + H2O
52.0% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
furoic acid + NH3
-
-
-
-
2-methyl-2-butenenitrile + H2O
14.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-methyl-2-butenoic acid + NH3
-
-
-
-
2-methyl-3-butenenitrile + H2O
46.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-methyl-3-butenoic acid + NH3
-
-
-
-
2-pentenenitrile + H2O
6.33% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-pentenoic acid + NH3
-
-
-
-
2-thiopheneacetonitrile + H2O
73.5% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
thiophene-2-carboxylate + NH3
-
-
-
-
2-thiophenecarbonitrile + H2O
61.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
2-thiophenecarboxylate + NH3
-
-
-
-
3,3'-oxydipropionitrile + H2O
29.0% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
? + NH3
-
-
-
-
3-chloropropionitrile + H2O
113% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-chloropropanoate + NH3
-
-
-
-
3-cyanopyridine + H2O
7.72% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-carboxypyridine + NH3
-
-
-
-
3-ethoxyacrylonitrile + H2O
19.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-ethoxyacrylic acid + NH3
-
-
-
-
3-ethoxyacrylonitrile + H2O
19.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
3-ethoxyacrylic acid + NH3
-
-
-
-
3-ethoxybenzonitrile + H2O
21.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-ethoxybenzoate + NH3
-
-
-
-
3-methoxybenzonitrile
24.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-methoxybenzoate + NH3
-
-
-
-
3-nitrobenzonitrile + H2O
74.5% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-nitrobenzoate + NH3
-
-
-
-
3-pentenenitrile + H2O
132% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-pentenoic acid + NH3
-
-
-
-
3-thiopheneacetonitrile + H2O
66.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
thiophene-3-carboxylate + NH3
-
-
-
-
3-tolunitrile + H2O
67.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
3-methylbenzenecarboxylic acid + NH3
-
-
-
-
3-xylylene dicyanide + H2O
14.62% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
?
-
-
-
-
4-chlorobutyronitrile + H2O
116% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
4-chlorobutyrate + NH3
-
-
-
-
4-cyano-1-cyclohexene + H2O
7.03% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
1-cyclohexene-4-carboxylate + NH3
-
-
-
-
4-cyanopyridine + H2O
9.71% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
4-carboxypyridine + NH3
-
-
-
-
acetonitrile + H2O
28.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
acetate + NH3
-
-
-
-
acrylonitrile + H2O
348% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
acrylic acid + NH3
-
-
-
-
acrylonitrile + H2O
348% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
acrylic acid + NH3
-
-
-
-
adiponitrile + H2O
110% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
adipic acid + NH3
-
-
-
-
benzonitrile + H2O
27.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
benzoate + NH3
-
-
-
-
butyronitrile + H2O
18.0% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
butyrate + NH3
-
-
-
-
capronitrile + H2O
38.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
hexanoate + NH3
-
-
-
-
chloroacetonitrile + H2O
61.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
chloroacetate + NH3
-
-
-
-
cinnamonitrile + H2O
31.2% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
cinnamate + NH3
-
-
-
-
crotonitrile + H2O
-
32506
Rhodococcus rhodochrous
crotonic acid + NH3
-
32506
Rhodococcus rhodochrous
-
crotonitrile + H2O
-
32506
Rhodococcus rhodochrous K22
crotonic acid + NH3
-
32506
Rhodococcus rhodochrous K22
-
cyanoacetic acid ethyl ester + H2O
117% of the activity withcrotononitrile
32506
Rhodococcus rhodochrous
acetic acid ethyl ester + NH3
-
-
-
-
cyanopyrazine + H2O
17.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
pyrazine-2-carboxylate + NH3
-
-
-
-
cyclopentanecarbonitrile + H2O
11.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
cyclopentanoic acid + NH3
-
-
-
-
cyclopropanecarbonitrile + H2O
22.9% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
cyclopropanoic acid + NH3
-
-
-
-
diaminomaleonitrile + H2O
18.6% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
diaminomaleic acid + NH3
-
-
-
-
fumaronitrile + H2O
27.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
fumaric acid + NH3
-
-
-
-
glutaronitrile + H2O
345% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
glutarate + NH3
-
-
-
-
iminodiacetonitrile + 2 H2O
261% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
iminodiacetic acid + 2 NH3
-
-
-
-
isobutyronitrile + H2O
6.74% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
isobutyrate + NH3
-
-
-
-
isocapronitrile + H2O
41.5% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
isohexanoate + NH3
-
-
-
-
isophthalonitrile + H2O
66.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
isophthalate + NH3
-
-
-
-
malononitrile + H2O
45.1% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
malonic acid + NH3
-
-
-
-
methacrylonitrile + H2O
143% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
methacrylic acid + NH3
-
-
-
-
methacrylonitrile + H2O
143% of the activity with crotononitrile
32506
Rhodococcus rhodochrous K22
methacrylic acid + NH3
-
-
-
-
methoxyacetonitrile + H2O
37.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
methoxyacetate + NH3
-
-
-
-
N-methyl-beta-alaninenitrile + H2O
11.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
N-methyl-beta-Ala + NH3
-
-
-
-
phenylacetonitrile + H2O
27.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
phenylacetate + NH3
-
-
-
-
pimelonitrile + H2O
27.3% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
pimelic acid + NH3
-
-
-
-
piperonylonitrile + H2O
6.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
(3,4-methylenedioxy)benzoate + NH3
-
-
-
-
propionitrile + H2O
12.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
propionate + NH3
-
-
-
-
sebaconitrile + H2O
15.7% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
? + NH3
-
-
-
-
suberonitrile + H2O
21.4% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
decanedioic acid + NH3
-
-
-
-
succinonitrile + H2O
271% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
succinate + NH3
-
-
-
-
valeronitrile + H2O
40.8% of the activity with crotononitrile
32506
Rhodococcus rhodochrous
pentanoate + NH3
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexadecamer
or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol
Rhodococcus rhodochrous
pentadecamer
or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol
Rhodococcus rhodochrous
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Rhodococcus rhodochrous
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
55
40°C: about 40% of maximal activity, 55°C: about 60% of maximal activity, crotonitrile
Rhodococcus rhodochrous
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
pH 7.5, 1 h, stable
Rhodococcus rhodochrous
30
-
pH 7.5, 1 h, 6% loss of activity
Rhodococcus rhodochrous
35
-
pH 7.5, 1 h, 8% loss of activity
Rhodococcus rhodochrous
40
-
pH 7.5, 1 h, 13% loss of activity
Rhodococcus rhodochrous
45
-
pH 7.5, 1 h, 72% loss of activity
Rhodococcus rhodochrous
50
-
pH 7.5, 1 h, 93% loss of activity
Rhodococcus rhodochrous
55
-
pH 7.5, 1 h, 100% loss of activity
Rhodococcus rhodochrous
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Rhodococcus rhodochrous
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
25°C, 45 min, less than 30% loss of activity
Rhodococcus rhodochrous
10
-
25°C, 45 min, 40% loss of activity
Rhodococcus rhodochrous
Other publictions for EC 3.5.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733526
Fang
Enzymatic degradation of aliph ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous BX2
Biores. Technol.
185
28-34
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733919
Yusuf
Cloning and functional charact ...
Fusarium proliferatum, Fusarium proliferatum AUF-2
Funct. Integr. Genomics
15
413-424
2015
1
-
1
-
-
-
12
1
-
6
1
-
-
2
-
-
1
-
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
12
-
1
-
6
1
-
-
-
-
1
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
734577
Zhang
Structural insights into enzym ...
Synechocystis sp.
J. Struct. Biol.
188
93-101
2014
-
-
1
1
25
-
-
18
-
-
-
-
-
1
-
-
1
-
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
18
-
-
-
-
-
-
-
1
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
735340
Wang
A novel nitrilase from Rhodoba ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides LHS-305
World J. Microbiol. Biotechnol.
30
245-252
2014
-
-
1
-
-
-
9
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
1
-
-
2
-
-
-
-
1
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
710984
Bayer
A nitrilase from a metagenomic ...
uncultured bacterium
Appl. Microbiol. Biotechnol.
89
91-98
2011
-
-
1
-
-
-
4
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
1
1
718661
He
A high-throughput screening st ...
Alcaligenes sp., Alcaligenes sp. ECU0401, Rhodococcus erythropolis, Rhodococcus erythropolis CGMCC 1.2362
Appl. Microbiol. Biotechnol.
89
817-823
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
695831
Kim
Identification and characteriz ...
Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5
Appl. Microbiol. Biotechnol.
83
273-283
2009
-
-
1
-
-
-
6
7
-
-
2
-
-
5
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
6
-
7
-
-
2
-
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
1
-
-
-
-
-
-
699065
Nigam
Nitrilase-catalysed conversion ...
Streptomyces sp.
J. Biosci.
34
21-26
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712260
Thuku
Microbial nitrilases: Versatil ...
Acidovorax facilis, Acidovorax facilis 72W, Acinetobacter sp., Acinetobacter sp. AK226, Comamonas testosteroni, Pseudomonas sp., Pseudomonas sp. S1, Pyrococcus abyssi, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechocystis sp.
J. Appl. Microbiol.
106
703-727
2009
1
2
-
-
3
-
-
-
-
-
14
-
-
22
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
1
2
-
-
-
3
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
-
-
-
712870
Sharma
-
In silico analysis of amino ac ...
Bradyrhizobium sp., Bradyrhizobium sp. ORS278, Methylibium petroleiphilum, Pseudomonas syringae pv. syringae, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechococcus elongatus
J. Proteomics Bioinform.
2
185-192
2009
-
-
-
-
-
-
-
-
-
-
6
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
685014
Yeom
A determinant residue of subst ...
Rhodococcus rhodochrous
Biochem. J.
415
401-407
2008
-
-
1
1
16
-
-
31
-
-
1
-
1
2
-
-
1
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
32
-
-
1
-
1
-
-
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
-
695708
Luo
Gene cloning, overexpression, ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6
Appl. Biochem. Biotechnol.
160
393-400
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687941
Khandelwal
-
Optimization of nitrilase prod ...
Streptomyces sp., Streptomyces sp. MTCC 7546
J. Chem. Technol. Biotechnol.
82
646-651
2007
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
-
-
-
670463
Bergeron
-
Nitrilase-catalyzed desymmetri ...
Pseudomonas fluorescens
Org. Proc. Res. Dev.
10
661-665
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670792
Mueller
Cloning, overexpression, and c ...
no activity in Aeropyrum pernix, no activity in Pyrococcus furiosus, no activity in Pyrococcus horikoshii, Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
Protein Expr. Purif.
47
672-681
2006
4
-
1
-
-
-
24
2
1
-
4
-
-
6
-
-
1
-
-
-
3
-
10
2
1
1
6
-
1
1
-
-
-
1
-
4
-
1
-
-
-
-
-
24
-
2
1
-
4
-
-
-
-
1
-
-
3
-
10
2
1
1
6
-
1
1
-
1
-
-
-
-
-
-
685425
Holtze
Transformation of the herbicid ...
Pseudomonas fluorescens 11387, Pseudomonas fluorescens, Pseudomonas putida 11388, Pseudomonas putida, Rhizobium sp. 11401, Rhizobium sp.
Biodegradation
17
503-510
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686545
Mukherjee
-
Exploring the synthetic applic ...
Synechocystis sp.
Eur. J. Org. Chem.
23
5238-5242
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670922
Hann
-
Regioselective biocatalytic hy ...
Acidovorax facilis, Acidovorax facilis 72W
Tetrahedron
60
577-581
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654298
Chauhan
Purification, cloning, sequenc ...
Acidovorax facilis, Acidovorax facilis 72W
Appl. Microbiol. Biotechnol.
61
118-122
2003
-
1
1
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655310
Brenner
Catalysis in the nitrilase sup ...
Arabidopsis thaliana, Rhodococcus rhodochrous
Curr. Opin. Struct. Biol.
12
775-782
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655149
Dias
Enzymatic degradation of nitri ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii UFMG-Y65
Can. J. Microbiol.
46
525-531
2000
-
2
1
-
-
-
1
-
1
-
-
-
-
2
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
1
-
2
1
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
209747
Dhillon
-
Transformation of aliphatic an ...
Pseudomonas sp., Pseudomonas sp. S1
Can. J. Microbiol.
45
811-815
1999
-
-
-
-
-
-
-
6
-
-
1
-
-
3
-
-
1
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
32507
Levy-Schil
Aliphatic nitrilase from a soi ...
Comamonas testosteroni
Gene
161
15-20
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
32505
Kobayashi
Primary structure of an alipha ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
Biochemistry
31
9000-9007
1992
-
-
1
-
2
-
-
-
-
-
1
-
-
10
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
32506
Kobayashi
Purification and characterizat ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
J. Bacteriol.
172
4807-4815
1990
-
-
-
-
-
-
3
2
-
1
3
-
-
6
-
-
1
-
-
-
1
-
63
2
1
1
7
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
1
3
-
-
-
-
1
-
-
1
-
63
2
1
1
7
-
1
-
2
-
-
-
-
-
-
-