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Literature summary for 3.5.5.5 extracted from

  • Thakur, N.; Kumar, V.; Thakur, S.; Sharma, N.; Sheeta, S.; Bhalla, T.
    Biotransformation of 4-hydroxyphenylacetonitrile to 4-hydroxyphenylacetic acid using whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 (2018), Proc. Biochem., 73, 117-123 .
No PubMed abstract available
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Application

Application Comment Organism
synthesis the whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 is employed to develop a bioprocess for the synthesis of 4-hydroxyphenylacetic acid from 4-hydroxyphenylacetonitrile. A fed batch process designed at 500 ml with five feedings of substrate results in 150 mM product formation using 18 U/ml whole cells nitrilase (16 mg/ml dcw) at 45°C and a volumetric and catalytic productivity of 23 g/l and 4.13 g/g dcw/h Alcaligenes faecalis

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyphenylacetic acid
-
 Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
20
-
 4-Hydroxyphenylacetonitrile pH 7.0, 30°C Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis MTCC 12629
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminoyphenylacetonitrile + H2O
-
 Alcaligenes faecalis ? + NH3
-
 ?
4-aminoyphenylacetonitrile + H2O
-
 Alcaligenes faecalis MTCC 12629 ? + NH3
-
 ?
4-hydroxyphenylacetonitrile + H2O
-
 Alcaligenes faecalis 4-hydroxyphenylacetic acid + NH3
-
 ?
4-hydroxyphenylacetonitrile + H2O
-
 Alcaligenes faecalis MTCC 12629 4-hydroxyphenylacetic acid + NH3
-
 ?
mandelonitrile + H2O
-
 Alcaligenes faecalis mandelic acid + NH3
-
 ?
mandelonitrile + H2O
-
 Alcaligenes faecalis MTCC 12629 mandelic acid + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Alcaligenes faecalis phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Alcaligenes faecalis MTCC 12629 phenylacetate + NH3
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life: 45 h Alcaligenes faecalis
45
-
 half-life: 36 h Alcaligenes faecalis
50
-
 half-life: 13 h Alcaligenes faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
18
-
 4-hydroxyphenylacetic acid pH 7.0, 30°C Alcaligenes faecalis

Expression

Organism Comment Expression
Alcaligenes faecalis isobutyronitrile emerges as the most appropriate inducer with a 1.2-fold enhanced nitrilase production in inducer feeding approach up