Literature summary for 3.5.5.5 extracted from

Sun, H.; Wang, H.; Gao, W.; Chen, L.; Wu, K.; Wei, D.
Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile (2015), Biochem. Biophys. Res. Commun., 468, 820-825 .

Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 (DE3)	Pseudomonas psychrotolerans

Protein Variants

Protein Variants	Comment	Organism
A136Y	mutant enzyme shows reversed selectivity and preferentially produces (R)-mandelic acid with an enantiomeric excess values of 66.7%	Pseudomonas psychrotolerans
A136Y/I168Y	mutant enzyme produces (R)-mandelic acid with an enantiomeric excess value of 89.7%, an R-enantioselectivity higher than that obtained with the single mutations A136Y and I168Y	Pseudomonas psychrotolerans
A136Y/I168Y/M113G	mutant enzyme with R-selectivity, 90.9% enantiomeric excess	Pseudomonas psychrotolerans
I168Y	mutant enzyme shows reversed selectivity and preferentially produces (R)-mandelic acid with an enantiomeric excess values of 74.3%	Pseudomonas psychrotolerans
M113C	mutation enhances the S-enantioselectivity of the enzyme toward mandelonitrile	Pseudomonas psychrotolerans
M113G/A136Y/I168Y	R-selectivity toward mandelonitrile with 90.1% enantiomeric excess	Pseudomonas psychrotolerans
M113L/R128H	S-selectivity toward mandelonitrile with 91.1% enantiomeric excess	Pseudomonas psychrotolerans
M113Q	mutation enhances the S-enantioselectivity of the enzyme toward mandelonitrile	Pseudomonas psychrotolerans
R128E	mutant displays no activity toward mandelonitrile	Pseudomonas psychrotolerans
R128H	mutation enhances the S-enantioselectivity of the enzyme toward mandelonitrile	Pseudomonas psychrotolerans
R128K	mutant demonstrates low enantioselectivity (44.9% enantiomeric excess) as compared with the wild-type enzyme (52.7% enantiomeric excess)	Pseudomonas psychrotolerans

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas psychrotolerans	-	-	-
Pseudomonas psychrotolerans L19	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R,S)-mandelonitrile + 2 H2O	the enzyme hydrolyzes racemic mandelonitrile to (S)-mandelic acid with an enantiomeric excess value of 52.7%	Pseudomonas psychrotolerans	(S)-mandelic acid + NH3	-	?
(R,S)-mandelonitrile + 2 H2O	the enzyme hydrolyzes racemic mandelonitrile to (S)-mandelic acid with an enantiomeric excess value of 52.7%	Pseudomonas psychrotolerans L19	(S)-mandelic acid + NH3	-	?

Synonyms

Synonyms	Comment	Organism
nitrilase PpL19	-	Pseudomonas psychrotolerans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Pseudomonas psychrotolerans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Pseudomonas psychrotolerans

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Release 2023.1 (January 2023)