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Literature summary for 3.5.5.5 extracted from

  • Thuku, R.; Brady, D.; Benedik, M.; Sewell, B.
    Microbial nitrilases: Versatile, spiral forming, industrial enzymes (2009), J. Appl. Microbiol., 106, 703-727.
    View publication on PubMed
Search for more literature for 3.5.5.5

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 14 * 32000 Alcaligenes faecalis
460000
-
-
 Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis ATCC 8750
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-mandelonitrile + 2 H2O
-
 Alcaligenes faecalis (R)-(-)mandelic acid + NH3
-
 ?
(R,S)-mandelonitrile + 2 H2O
-
 Alcaligenes faecalis ATCC 8750 (R)-(-)mandelic acid + NH3
-
 ?
4-aminobenzylcyanide + H2O high activity Alcaligenes faecalis 4-aminophenylacetic acid + NH3
-
 ?
4-aminobenzylcyanide + H2O high activity Alcaligenes faecalis ATCC 8750 4-aminophenylacetic acid + NH3
-
 ?
benzonitrile + 2 H2O low activity Alcaligenes faecalis benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O low activity Alcaligenes faecalis ATCC 8750 benzoic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
homotetradecamer 14 * 32000 Alcaligenes faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Alcaligenes faecalis