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Literature summary for 3.5.5.1 extracted from

  • Chen, H.; Zhang, T.; Sun, T.; Ni, Z.; Le, Y.; Tian, R.; Chen, Z.; Zhang, C.
    Clostridium thermocellum nitrilase expression and surface display on Bacillus subtilis spores (2015), J. Mol. Microbiol. Biotechnol., 25, 381-387 .
    View publication on PubMed
Search for more literature for 3.5.5.1

Application

Application Comment Organism
industry spore surface display of nitrilases is an effective approach for enzyme immobilization in biochemical engineering on an industrial scale Acetivibrio thermocellus

Cloned(Commentary)

Cloned (Comment) Organism
expressed, identified and displayed on the surface of Bacillus subtilis spores by using the spore coat protein G of Bacillus subtilis as an anchoring motif Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 SDS-PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DHT2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acetivibrio thermocellus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + H2O
-
 Acetivibrio thermocellus pyridine 3-carboxylic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Acetivibrio thermocellus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 35% of maximal activity, 50°C: about 55% of maximal activity Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Acetivibrio thermocellus