Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Alcaligenes sp. |
General Stability | Organism |
---|---|
the recombinant nitrilase (from Escherichia coli strain JM109/pNLE) is quite stable, the residual activity after 10 cycles of reaction is still over 40% of the initial activity | Alcaligenes sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.8 | - |
mandelonitrile | at 30°C and pH 8.0 | Alcaligenes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
x * 38500, calculated from amino acid sequence | Alcaligenes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | C5IIS9 | - |
- |
Alcaligenes sp. ECU0401 | C5IIS9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.6 | - |
purified enzyme, using mandelonitrile as substrate, at 30°C and pH 8.0 | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mandelonitrile + 2 H2O | - |
Alcaligenes sp. | (R)-mandelic acid + NH3 | - |
? | |
mandelonitrile + 2 H2O | - |
Alcaligenes sp. ECU0401 | (R)-mandelic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38500, calculated from amino acid sequence | Alcaligenes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Alcaligenes sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | - |
Alcaligenes sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme is quite stable at 30°C, with half-life of 63 h, the enzyme is labile at higher temperatures as revealed by half-lives of 35 h and 22 h at 40°C and 50°C, respectively | Alcaligenes sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
175 | - |
mandelonitrile | at 30°C and pH 8.0 | Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Alcaligenes sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.6 | the nitrilase activity was low at pH less 7.0 or pH above 8.6 | Alcaligenes sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
mandelonitrile | at 30°C and pH 8.0 | Alcaligenes sp. |