Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
General Stability | Organism |
---|---|
nitrilase aggregates are prepared using ammonium sulfate (35%) precipitation followed by cross-linking with glutaraldehyde (125 mM) which renders 70% activity retention | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida MTCC 5110 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
35% (w/v) ammonium sulfate precipitation | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mandelonitrile + 2 H2O | - |
Pseudomonas putida | mandelic acid + NH3 | - |
? | |
mandelonitrile + 2 H2O | - |
Pseudomonas putida MTCC 5110 | mandelic acid + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
activity of cross-linked enzyme aggregate preparation remains stable up to 75 h at 4°C while the free enzyme loses 90% activity at this temperature in 75 h | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the activity in the CLEA increases with the increase in the pH of cross-linking solution and it is maximum at pH 7.0 | Pseudomonas putida |