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Literature summary for 3.5.5.1 extracted from

  • Banerjee, A.; Dubey, S.; Kaul, P.; Barse, B.; Piotrowski, M.; Banerjee, U.C.
    enantioselective nitrilase from Pseudomonas putida: cloning, heterologous expression, and bioreactor studies (2008), Mol. Biotechnol., 41, 35-41.
    View publication on PubMed
Search for more literature for 3.5.5.1

Application

Application Comment Organism
synthesis synthesis of (R)-mandelic acid from (R,S)-mandelonitrile by use of Escherichia coli overexpressing the enzyme in a bioreactor. After a single batch reaction, (R)-mandelic acid can be obtained with 87% yield and 99.99% enantiomeric excess Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.26
-
 pH 7.5 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (R,S)-mandelonitrile + 4 H2O
-
 Pseudomonas putida (R)-mandelic acid + NH3 + (S)-mandelonitrile
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pseudomonas putida