Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) pLysS cells | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
2 * 36500, inactive enzyme, reducing SDS-PAGE | Rhodococcus rhodochrous |
40000 | - |
12 * 40000, active enzyme form, gel filtration | Rhodococcus rhodochrous |
480000 | - |
gel filtration | Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
- |
- |
Rhodococcus rhodochrous J1 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant enzyme undergoes posttranslational cleavage at approximately residue 327, resulting in the formation of active, helical homo-oligomers | Rhodococcus rhodochrous |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Sephacryl S-400 gel filtration | Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous J1 | benzoic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36500, inactive enzyme, reducing SDS-PAGE | Rhodococcus rhodochrous |
homododecamer | 12 * 40000, active enzyme form, gel filtration | Rhodococcus rhodochrous |