Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-beta-D-arabinofuranosylcytosine 5'-triphosphate | low activation, allosteric activator | Homo sapiens | |
CDP | low activation, allosteric activator | Homo sapiens | |
CTP | low activation, allosteric activator | Homo sapiens | |
dAMP | 0.025 mM: low activation | Homo sapiens | |
dCDP | low activation, allosteric activator | Homo sapiens | |
dCTP | allosteric activator | Homo sapiens | |
dCTP | less than 0.001 mM: very high activation | Homo sapiens | |
dCTP | activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP | Homo sapiens | |
TTP | activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition | Homo sapiens |
General Stability | Organism |
---|---|
dCTP and MgCl2 stabilize | Homo sapiens |
dithiothreitol, 2 mM, stabilizes | Homo sapiens |
glycerol, 15%, stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | slight inhibition | Homo sapiens | |
AMP | slight inhibition, competitive inhibitor, mechanism of inhibition | Homo sapiens | |
Co2+ | 1 mM: 75% inhibition | Homo sapiens | |
Cu2+ | 1 mM: 98% inhibition | Homo sapiens | |
dGDP | slight inhibition | Homo sapiens | |
dGMP | effective competitive inhibitor, mechanism of inhibition | Homo sapiens | |
dUMP | poor competitive inhibitor, mechanism of inhibition | Homo sapiens | |
dUTP | slight inhibition | Homo sapiens | |
EDTA | no inhibition by EDTA | Homo sapiens | |
GMP | slight inhibition | Homo sapiens | |
additional information | no inhibition by EDTA | Homo sapiens | |
TDP | slight inhibition, competitive inhibitor, mechanism of inhibition | Homo sapiens | |
TMP | poor competitive inhibitor, mechanism of inhibition | Homo sapiens | |
TTP | activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP | Homo sapiens | |
UTP | slight inhibition | Homo sapiens | |
Zn2+ | 1 mM: 94% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic behavior and data | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP | Homo sapiens | |
Mg2+ | activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP | Homo sapiens | |
Mn2+ | activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP | Homo sapiens | |
additional information | metal ions not required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Homo sapiens |
109500 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCMP + H2O | Homo sapiens | important role in regulation of intracellular TTP levels | dUMP + NH3 | - |
ir | |
dCMP + H2O | Homo sapiens | enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP | dUMP + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Homo sapiens | |
dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-50°C, 0.05 mM dCTP, 1 mM MgCl2 or 2 mM dithiothreitol, 18 h, 40% activity lost | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ara-CMP + H2O | arabinosyl-CMP | Homo sapiens | ara-UMP + NH3 | - |
? | |
ara-CMP + H2O | 1-beta-D-arabinofuranosyl-CMP | Homo sapiens | ara-UMP + NH3 | - |
? | |
ara-CMP + H2O | effective substrate in presence of dCTP | Homo sapiens | ara-UMP + NH3 | - |
? | |
CMP + H2O | poor substrate | Homo sapiens | UMP + NH3 | - |
? | |
dCMP + H2O | high degree of specificity of dCMP | Homo sapiens | dUMP + NH3 | - |
ir | |
dCMP + H2O | important role in regulation of intracellular TTP levels | Homo sapiens | dUMP + NH3 | - |
ir | |
dCMP + H2O | enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP | Homo sapiens | dUMP + NH3 | - |
ir | |
additional information | substrate specificity | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.10 | Homo sapiens |
6.5 | 7.3 | broad pH optimum | Homo sapiens |
7.2 | - |
assay at | Homo sapiens |