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Literature summary for 3.5.3.7 extracted from

  • Lee, S.J.; Kim, d.o..J.; Kim, H.S.; Lee, B.I.; Yoon, H.J.; Yoon, J.Y.; Kim, K.H.; Jang, J.Y.; Im, H.N.; An, D.R.; Song, J.S.; Kim, H.J.; Suh, S.W.
    Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily (2011), J. Struct. Biol., 175, 329-338.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of Pseudomonas aeruginosa guanidinobutyrase (GbuA) and guanidinopropionase (GpuA) are determined. Although GbuA and GpuA share a common structural fold of the typical ureohydrolase superfamily, they exhibit significant variations in two active site loops Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
M161Y mutant showed no detectable enzymatic activity against guanidium compounds Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.7
-
4-Guanidinobutyrate pH 7.9, 30°C, wild-type Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Guanidinobutyrate + H2O enzyme shows no activity towards 3-guanidinopropionate Pseudomonas aeruginosa 4-Aminobutyrate + urea
-
?

Synonyms

Synonyms Comment Organism
GbuA
-
Pseudomonas aeruginosa
guanidinobutyrase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
94.7
-
4-Guanidinobutyrate pH 7.9, 30°C, wild-type Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Pseudomonas aeruginosa