Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
crystal structures of Pseudomonas aeruginosa guanidinobutyrase (GbuA) and guanidinopropionase (GpuA) are determined. Although GbuA and GpuA share a common structural fold of the typical ureohydrolase superfamily, they exhibit significant variations in two active site loops | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
M161Y | mutant showed no detectable enzymatic activity against guanidium compounds | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
4-Guanidinobutyrate | pH 7.9, 30°C, wild-type | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Guanidinobutyrate + H2O | enzyme shows no activity towards 3-guanidinopropionate | Pseudomonas aeruginosa | 4-Aminobutyrate + urea | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GbuA | - |
Pseudomonas aeruginosa |
guanidinobutyrase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
94.7 | - |
4-Guanidinobutyrate | pH 7.9, 30°C, wild-type | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Pseudomonas aeruginosa |