Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arcaine | competitive inhibitor | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
agmatine | - |
Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43500 | - |
1 * 43500 + 1 * 44000, SDS-PAGE | Zea mays |
44000 | - |
1 * 43500 + 1 * 44000, SDS-PAGE | Zea mays |
75000 | - |
gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme purified from shoots grown in the dark by DE52 column chromatography, Sephadex G-100 column chromatography and agmatine-epoxy-Sepharose 6B column chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
crude extract of enzyme | Zea mays |
0.015 | - |
enzyme activity of shoots grown for 7 days at 25°C in the dark and light respectively | Zea mays |
32.2 | - |
purified enzyme | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | - |
Zea mays | N-carbamoylputrescine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 43500 + 1 * 44000, SDS-PAGE | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
purified enzyme stable for two months | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
purified enzyme shows 85% of maximum activity | Zea mays |
8 | - |
purified enzyme shows 85% of maximum activity | Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
arcaine | - |
Zea mays |