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Literature summary for 3.5.2.B3 extracted from
- Characterization of a recombinant (-)gamma-lactamase from Microbacterium hydrocarbonoxydans (2012), Biotechnol. Lett., 34, 275-279.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 1 mM, 130% of initial activity | Microbacterium hydrocarbonoxydans |  |
| EDTA | 1 mM, 110% of initial activity | Microbacterium hydrocarbonoxydans | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Microbacterium hydrocarbonoxydans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the catalytic triad of the (-)-gamma-lactamase is comprised of residues Ser98, Asp230, and His259 and an oxyanion hole is formed by Tyr32 and Met99 according to the alignment results | Microbacterium hydrocarbonoxydans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31300 | - |
2 * 31300, calculated, 2 * 33000, SDS-PAGE | Microbacterium hydrocarbonoxydans |
| 33000 | - |
2 * 31300, calculated, 2 * 33000, SDS-PAGE | Microbacterium hydrocarbonoxydans |
| 65000 | - |
PAGE | Microbacterium hydrocarbonoxydans |
| 65300 | - |
gel filtration | Microbacterium hydrocarbonoxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Microbacterium hydrocarbonoxydans | E3SVS0 | additionally acts as amidase, EC 3.5.1.4 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1R,4S)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O | - |
Microbacterium hydrocarbonoxydans | (1S,4R)-4-aminocyclopent-2-ene-1-carbaldehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31300, calculated, 2 * 33000, SDS-PAGE | Microbacterium hydrocarbonoxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (-)-gamma-lactamase | - |
Microbacterium hydrocarbonoxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Microbacterium hydrocarbonoxydans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
half-life 30 min | Microbacterium hydrocarbonoxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Microbacterium hydrocarbonoxydans |
| 8 | - |
- |
Microbacterium hydrocarbonoxydans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Microbacterium hydrocarbonoxydans | calculated | - |
4.7 |
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