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Literature summary for 3.5.2.20 extracted from

  • Sommer, T.; Bjerregaard-Andersen, K.; Simensen, S.M.; Jensen, J.K.; Jochimsen, B.; Riss, P.J.; Etzerodt, M.; Morth, J.P.
    Enzymatic detection and quantification assay of isatin, a putative stress biomarker in blood (2015), ACS Chem. Neurosci., 6, 1353-1360 .
    View publication on PubMed
Search for more literature for 3.5.2.20

Application

Application Comment Organism
analysis a fluorescence-based enzymatic assay is developed that can quantify isatin, a putative stress biomarkerin blood samples. A phase extraction of isatin followed by a second phase extraction combined with an enzymatic reaction performed by an isatin hydrolase is used to extract and quantify isatin in whole blood samples Roseibium aggregatum

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly produced in Escherichia coli Roseibium aggregatum

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 35% of the activity compared to Mn2+ Roseibium aggregatum
Mn2+ the enzyme is highly selective for Mn2+ with a dissociation constant determined to be 0.0095 mM Roseibium aggregatum
Ni2+ 30% of the activity compared to Mn2+ Roseibium aggregatum

Organism

Organism UniProt Comment Textmining
Roseibium aggregatum A0NLY7
-
-
Roseibium aggregatum ATCC 25650 A0NLY7
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isatin + H2O
-
 Roseibium aggregatum isatinate
-
 ?
isatin + H2O
-
 Roseibium aggregatum ATCC 25650 isatinate
-
 ?

Synonyms

Synonyms Comment Organism
IH-b
-
 Roseibium aggregatum
isatin hydrolase b
-
 Roseibium aggregatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 t1/2: 9 min Roseibium aggregatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Roseibium aggregatum

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 50% of maximal activity, pH 9.5: about 55% of maximal activity Roseibium aggregatum