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Literature summary for 3.5.2.19 extracted from
- The biological function of the bacterial isochorismatase-like hydrolase SttH (2009), Biosci. Biotechnol. Biochem., 73, 2494-2500.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces albulus |
| expression in Escherichia coli | Streptomyces noursei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C158S | no activity detected | Streptomyces noursei |
| C176S | no activity detected | Streptomyces albulus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
streptothricin-F | pH 6.5, 30°C | Streptomyces noursei |  |
| 3.1 | - |
streptothricin-F | pH 6.5, 30°C | Streptomyces albulus | |
| 3.2 | - |
streptothricin-D | pH 6.5, 30°C | Streptomyces noursei | |
| 17.2 | - |
streptothricin-D | pH 6.5, 30°C | Streptomyces albulus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| streptothricin-D + H2O | Streptomyces noursei | - |
streptothricin-D acid | - |
? | |
| streptothricin-D + H2O | Streptomyces noursei NBRC15452 | - |
streptothricin-D acid | - |
? | |
| streptothricin-F + H2O | Streptomyces noursei | - |
streptothricin-F acid | - |
? | |
| streptothricin-F + H2O | Streptomyces noursei NBRC15452 | - |
streptothricin-F acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces albulus | - |
- |
- |
| Streptomyces albulus NBRC14147 | - |
- |
- |
| Streptomyces noursei | C5NU54 | - |
- |
| Streptomyces noursei NBRC15452 | C5NU54 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| streptothricin-D + H2O | - |
Streptomyces noursei | streptothricin-D acid | - |
? | |
| streptothricin-D + H2O | - |
Streptomyces albulus | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| streptothricin-D + H2O | - |
Streptomyces noursei | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| streptothricin-D + H2O | - |
Streptomyces albulus NBRC14147 | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| streptothricin-D + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-D acid | - |
? | |
| streptothricin-D + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| streptothricin-F + H2O | - |
Streptomyces noursei | streptothricin-F acid | - |
? | |
| streptothricin-F + H2O | - |
Streptomyces albulus | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
| streptothricin-F + H2O | - |
Streptomyces noursei | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
| streptothricin-F + H2O | - |
Streptomyces albulus NBRC14147 | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
| streptothricin-F + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-F acid | - |
? | |
| streptothricin-F + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sttH | - |
Streptomyces albulus |
| sttH | - |
Streptomyces noursei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Streptomyces albulus |
| 55 | - |
- |
Streptomyces noursei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces albulus |
| 7 | - |
- |
Streptomyces noursei |
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