Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in Tris/HCl buffer, optimum concentration of NaCl is 50 mM for isoforms HD1 and HD2 and 100 mM for the nuclear matrix form | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Butyrate | non-competitive | Gallus gallus | |
additional information | in phosphate buffer, the presence of NaCl is inhibitory | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nuclear matrix | associated to | Gallus gallus | 16363 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
isoform HD1, gel filtration | Gallus gallus |
220000 | - |
isoform HD2, gel filtration | Gallus gallus |
400000 | - |
enzyme complex solubilized from nuclear matrix, gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
isoforms HD1 and HD2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-lysine acetylated histone H2A-H2B dimer + H2O | isoform HD2, slightly preferred substrate | Gallus gallus | histone H3-H4 dimer + acetate | - |
? | |
N6-lysine acetylated histone H3-H4 tetramer + H2O | isoform HD1, preferred substrate | Gallus gallus | histone H3-H4 tetramer + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | isoform HD2 dissociates into HD1 when treated with 1.6 M NaCl or applied to a Q-Sepharose column. The active form of enzyme is a high-molecular-mass complex associated with proteins that are components of the nuclear matrix | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | isoform HD2 | Gallus gallus |
7 | 7.5 | nuclear matrix form of enzyme | Gallus gallus |
7.5 | - |
isoform HD1 | Gallus gallus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.021 | - |
isoform HD2 | Gallus gallus | Butyrate | |
0.045 | - |
isoform HD1 | Gallus gallus | Butyrate | |
0.08 | - |
nuclear matrix form of enzyme | Gallus gallus | Butyrate |