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Literature summary for 3.5.1.97 extracted from
- Interference of quorum sensing by Delftia sp. VM4 depends on the activity of a novel N-acylhomoserine lactone-acylase (2015), PLoS ONE, 10, e0138034 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis of isolate VM4 within the genus Delftia | Delftia sp. VM4 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 26% inhibition at 1 mM | Delftia sp. VM4 |  |
| Cd2+ | complete inhibition at 1 mM | Delftia sp. VM4 | |
| Cu2+ | complete inhibition at 1 mM | Delftia sp. VM4 | |
| Mn2+ | 37% inhibition at 1 mM | Delftia sp. VM4 | |
| Ni2+ | 48% inhibition at 1 mM | Delftia sp. VM4 | |
| Pb2+ | complete inhibition at 1 mM | Delftia sp. VM4 | |
| Zn2+ | 73% inhibition at 1 mM | Delftia sp. VM4 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics and thermodynamics | Delftia sp. VM4 | |
| 0.5 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
| 0.67 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
| 1.25 | - |
N-hexanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect on enzyme activity by 2 mM of Na+, K+, Mg2+, and Ca2+ | Delftia sp. VM4 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
gel filtration, and mass spectrometry MALDI-TOF | Delftia sp. VM4 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-3-oxo-hexanoyl-L-homoserine lactone + H2O | Delftia sp. VM4 | - |
L-homoserine lactone + 3-oxohexanoate | - |
? | |
| N-3-oxo-octanoyl-L-homoserine lactone + H2O | Delftia sp. VM4 | - |
L-homoserine lactone + 3-oxo-octanoate | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | Delftia sp. VM4 | - |
L-homoserine lactone + decanoate | - |
? | |
| N-hexanoyl-L-homoserine lactone + H2O | Delftia sp. VM4 | - |
L-homoserine lactone + hexanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Delftia sp. VM4 | - |
isolated from turf soil | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 155.8fold from strain VM4 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, ultrafiltration, and gel filtration | Delftia sp. VM4 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the culture medium pH of Delftia sp. strain VM4 increases from pH 7.2 to pH 7.8 within 6 h | Delftia sp. VM4 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | screening for the AHL-degrading properties in AHL-bioassay with biosensor strain Chromobacterium violaceum CV026 | Delftia sp. VM4 | ? | - |
- |
|
| N-3-oxo-hexanoyl-L-homoserine lactone + H2O | - |
Delftia sp. VM4 | L-homoserine lactone + 3-oxohexanoate | - |
? | |
| N-3-oxo-octanoyl-L-homoserine lactone + H2O | - |
Delftia sp. VM4 | L-homoserine lactone + 3-oxo-octanoate | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | - |
Delftia sp. VM4 | L-homoserine lactone + decanoate | - |
? | |
| N-hexanoyl-L-homoserine lactone + H2O | - |
Delftia sp. VM4 | L-homoserine lactone + hexanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | peptide mass fingerprinting analysis of the active protein band (from native PAGE) possessing AHL degrading activity, peptide sequence comparisons, overview | Delftia sp. VM4 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AHL acylase | - |
Delftia sp. VM4 |
| N-acylhomoserine lactone-acylase | - |
Delftia sp. VM4 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | - |
Delftia sp. VM4 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22.8 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
| 24.3 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
| 33.2 | - |
N-hexanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Delftia sp. VM4 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme shows an AHL acylase type mode of action. It possesses similarity with an alpha/beta-hydrolase fold protein, which makes it unique among the known AHL acylases with domains of the N-terminal nucleophile (Ntn)-hydrolase superfamily. Phylogenetic analysis of isolate VM4 within the genus Delftia | Delftia sp. VM4 |
| metabolism | virulence gene expression in many Gram-negative pathogens generally is under quorum sensing control. In particular, quorum-sensing plays an important role in the regulation of virulence genes (mainly plant cell wall degrading enzymes, PCWDEs, production) in phytopathogenic soft rot causing Pectobacterium species. Also the N-acylhomoserine lactone (AHL) based quorum sensing in soft rot Pectobacterium species tightly regulates the activities implicated in microbial competition and host-pathogen interaction. These activities require various microbial functions such as biofilm formation, expression of virulence factors, antibiotic production etc. | Delftia sp. VM4 |
| additional information | Delftia sp. VM4 enzyme structure model of alpha/beta-hydrolase fold protein, overview | Delftia sp. VM4 |
| physiological function | Delftia sp. VM4 can degrade exogenous N-acyl homoserine lactone (AHL), hence it effectively attenuates the virulence of bacterial soft rot pathogen Pectobacterium carotovorum subsp. carotovorum strain BR1 (Pcc BR1) as a consequence of quorum sensing inhibition. Interference of quorum sensing by Delftia sp. VM4 depends on the activity of the N-acylhomoserine lactone-acylase. Delftia sp. VM4 interferes with quorum-sensing signaling of Pcc BR1 when they live commensally in vitro, and such signal interruption causes drastic attenuation of Pcc BR1 virulence determinants. Delftia sp. VM4 cells do not produce an antibiotic-like material to hinder the growth of Pcc BR1 | Delftia sp. VM4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.56 | - |
N-hexanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
| 32.27 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
| 45.6 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 6.5, 30°C | Delftia sp. VM4 | |
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